通过代谢工程和生物工艺优化,通过Lipomyces starkeyi的玉米水解液使酸产生
Jeffrey J Czajka1,2, Ziyu Dai1,2, Tijana Radivojević2,3,4
1Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
Microbial cell factories
|May 20, 2025
概括
这项研究利用油性酵母Lipomyces starkeyi从废弃原料中产生酸,在生物反应器中达到26.5g/L. 这表明了生产有机酸具有最小副产品的新能力.
科学领域:
- 微生物学 微生物学
- 代谢工程是代谢工程.
- 生物技术是生物技术.
背景情况:
- Lipomyces starkeyi是一种适用于生物燃料和废物化学品生产的油性酵母.
- 基因工程的进步使得L. starkeyi的发展成为可能,但对其新陈代谢转向其他产品还没有充分探索.
研究的目的:
- 通过引入还原性TCA通路和C4-二碳酸转运器来设计L. starkeyi用于酸生产.
- 探索将脂质流转向其他有价值产品的可行性.
主要方法:
- 马拉酸转运体和马拉酸脱酶的异质表达.
- 过度表达的原生酸盐碳酸酶.
- 在瓶和受控生物反应器中使用玉米水解剂发酵.
- 蛋白质组,转录组和代谢组分析.
- 基于机器学习的媒介优化.
主要成果:
- 达到了L. starkeyi酸的产量,在瓶中达到大约10g/L.
- 在pH值<4.4时,酸的产生被抑制.
- 在控制的生物反应器发酵中,在玉米炉水解剂上产生26.5g/L的酸.
- 确定了与应激反应和在真实水解中运输相关的上调基因和蛋白质.
- 优化的介质提高了18%的产量,并显示出较低的耐受性.
结论:
- 在L. starkeyi中成功生产有机酸,并产生最小的副产品.
- 为了解L. starkeyi生理学和对水解成长的反应提供了丰富的数据集.
- 在未来的工程工作中确定了改善生长和耐受性的潜在基因标.
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