相关实验视频

Updated: May 23, 2025

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

削减Mycobacterium结核病复制:TRIM32对于细菌无处不在和巨细胞的自诱导是必要的

Alessandra Romagnoli¹, Martina Di Rienzo¹, Mauro Piacentini^1,2

¹Department of Epidemiology, Preclinical Research and Advanced Diagnostics, National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy.

Autophagy reports

|May 21, 2025

概括

三方基因 (TRIM) 蛋白调节自和免疫. TRIM32增强了自杀死Mycobacterium结核病 (Mtb),而TRIM36和TRIM56促进了Mtb的复制,提供了新的治疗点.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
微生物学微生物学

背景情况:

结核菌 (Mycobacterium tuberculosis,Mtb) 通过抑制细胞内关键过程 - - 自而逃避宿主防御.
自增强可以触发mtb杀死和免疫反应,使其成为治疗目标.
三方基因 (TRIM) 蛋白质是参与天生的免疫力,炎症和自调节的E3泛素连接酶.

研究的目的:

为了识别调节Mtb复制的TRIM蛋白.
阐明TRIM蛋白质影响Mtb细胞内生存的分子机制.
探索针对宿主导的Mtb疗法的TRIM蛋白的潜在向.

主要方法:

转录和感染性选以确定影响Mtb复制的TRIM.
在THP1巨细胞中对特定TRIM蛋白的过度表达和下调研究.
对mtb无处不在的分析,异种的标志物 (CALCOCO2 / NDP52,MAP1LC3B) 和mtb复制 (殖民地形成单位).

主要成果:

过度表达TRIM22和TRIM32减少了Mtb的生长,而TRIM36和TRIM56则促进了它的生长.
TRIM32过度表达通过增加Mtb无处不在和CALCOCO2/NDP52和MAP1LC3B的招募来增强外食.
降低TRIM32的调节损害了外来食物和增加了Mtb的复制.

结论:

在宿主对MTB感染的仇外反应中,TRIM32起着至关重要的作用.
TRIM36和TRIM56被确定为支持Mtb复制的宿主因素.
TRIM蛋白质代表了针对结核病的新型宿主导疗法的潜在目标.

关键词:

结核病菌菌菌的结核病菌的结核病菌.在TRIM中,我们使用了ubiquitin结合酶.乌比奎丁是一种存在的药物.异国主义是异国主义.

更多相关视频

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Published on: September 18, 2020

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

相关概念视频

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

The Unfolded Protein Response

The Unfolded Protein Response

The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...

Export of Misfolded Proteins out of the ER

Export of Misfolded Proteins out of the ER

After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...