Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

Search research articles

相关实验视频

Updated: May 23, 2025

Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging

Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging

Published on: November 21, 2023

在鼠标皮质中开发一个Ministroke模型

Song Wang¹, Sichen Wang², Yuhan Sun²

¹Experimental and Translational Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

|May 21, 2025

概括

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Zscan4c activates endogenous retrovirus MERVL and cleavage embryo genes.

Nucleic acids research·2019

Same author

Kinesin family member C1 accelerates bladder cancer cell proliferation and induces epithelial-mesenchymal transition via Akt/GSK3β signaling.

Cancer science·2019

Same author

MSI2-TGF-β/TGF-β R1/SMAD3 positive feedback regulation in glioblastoma.

Cancer chemotherapy and pharmacology·2019

Same author

METTL3 facilitates tumor progression via an m<sup>6</sup>A-IGF2BP2-dependent mechanism in colorectal carcinoma.

Molecular cancer·2019

Same author

Association between suicide and multiple sclerosis: An updated meta-analysis.

Multiple sclerosis and related disorders·2019

Same author

The Open Chromatin Landscape of Non-Small Cell Lung Carcinoma.

Cancer research·2019

研究人员在小鼠身上开发了一种新型的Ministroke模型,针对大脑皮层. 这种可复制的模型为研究轻微中风和中风复发的研究提供了有价值的工具,死亡率最低.

科学领域:

神经科学是一个神经科学.
神经学神经学
缺血性中风研究

背景情况:

脑卒中是全球死亡和残疾的主要原因,缺血性脑卒中占病例的85%.
目前用于中风的动物模型,例如中脑动脉阻塞 (MCAO),通常会产生可变和大损伤体积,限制它们在研究特定中风机制方面的实用性.
需要精细的动物模型,准确地模仿人类中风病理学,以有效研究大脑损伤机制.

研究的目的:

在小鼠中引入和验证一种新型的 ministroke 模型,专门针对 barrel 皮质.
建立一个可复制的模型,用于研究轻微中风和中风复发背后的分子机制.
通过敏感的行为测试来评估功能结果.

主要方法:

在小鼠中,右中脑动脉 (MCA) 的远端分支的永久绑定.
双侧常见大脑动脉 (CCA) 暂时封闭7分钟,然后再输血.
在感觉运动皮质,特别是皮质皮质的局部缺血的诱导.

主要成果:

开发的ministeroke模型始终产生很小的心脏病发作量,死亡率非常低.
该模型允许精确准大脑皮层,促进对局部脑损伤的研究.
震动引起的前肢放置试验和粘合剂去除试验在评估功能缺陷方面表现出高灵敏度.

关键词:

桶皮质的皮质是指桶皮质的皮质.缺血性中风是因为缺血性中风.长期评估长期评估死亡率较低,死亡率较低.澳大利亚MCAOO 部长的打击部长的打击部长

更多相关视频

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Published on: July 31, 2014

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Published on: January 6, 2011

相关实验视频

Last Updated: May 23, 2025

Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging

Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging

Published on: November 21, 2023

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Published on: July 31, 2014

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Published on: January 6, 2011

结论:

这种ministroke模型为研究轻微中风和中风复发提供了一个可重复和可靠的平台.
该模型能够产生小,一致的病变和低死亡率,这支持长期功能性结果评估.
目标性脑皮层缺血,结合敏感的行为测试,为中风研究提供了有价值的工具.