长非编码RNAMALAT1可以通过向miR-23a-3p通过调节ERK信号调节来调节LPS治疗的HK-2细胞的增殖和亡
Jinxia Li1, Haibo Liu2, Chao Li2
1Department of Critical Care, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
Archives of medical science : AMS
|May 21, 2025
概括
长非编码RNA MALAT1 在败血症引起的急性损伤中升高. 马拉特1通过miR-23a-3p和ERK信号通路调节细胞增殖和细胞亡,提供潜在的治疗点.
科学领域:
- 分子生物学分子生物学
- 脏生理学 脏生理学
背景情况:
- 败血症引起的急性损伤 (败血症AKI) 是一种具有高死亡率的危急病.
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在各种疾病中的作用,包括AKI.
研究的目的:
- 为了研究 lncRNA MALAT1 在败血症 AKI 中的作用.
- 阐明涉及miR-23a-3p和ERK信号的潜在分子机制.
主要方法:
- 来自性AKI患者和健康对照者的血清样本被分析到MALAT1水平.
- 使用LPS治疗的HK-2细胞进行的体外实验评估了MALAT1和miR-23a-3p对细胞增殖和细胞亡的影响.
- 研究了ERK信号的参与.
主要成果:
- 在性AKI患者和用LPS治疗的细胞中,MALAT1水平显著增加.
- 过度表达MALAT1减轻了LPS诱导的细胞损伤.
- 通过激活ERK信号传递,MALAT1被证实准miR-23a-3p,MALAT1siRNA促进细胞增殖并抑制细胞亡.
- 击败miR-23a-3p部分扭转了MALAT1siRNA的抗亡作用.
结论:
- 马拉特1 / miR-23a-3p轴在调节性AKI中的增殖和亡中发挥着至关重要的作用.
- 这个轴调节ERK信号传输,表明它是性AKI的潜在治疗标.
相关概念视频
lncRNA - Long Non-coding RNAs
8.4K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.4K
PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K
Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Interactions Between Signaling Pathways
6.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
MAPK Signaling Cascades
5.1K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.1K


