相关实验视频
Updated: May 23, 2025

10:54
A Cell Culture Model of Resistance Arteries
Published on: September 8, 2017
7.8K
抵抗的艺术 抵抗的艺术
1Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, 560064, India.
Autophagy reports
|May 21, 2025
概括
疟疾寄生虫中的阿尔特米西宁耐药性与细胞过程自有关. 这项研究表明,阻断自可以作为对抗耐药疟疾的策略.
科学领域:
- 分子寄生虫学 分子寄生虫学
- 药物耐药性机制 药物耐药性机制
- 细胞应激反应细胞应激反应
背景情况:
- *P. falciparum*对艺术素 (ART) 的耐药性是一个越来越令人担忧的问题,特别是在东南亚.
- 凯尔赫13和*Pf*ATG18的突变与ART耐药性有关.
- 自在寄生虫蛋白质稳定和ART耐药性中的作用仍然未经实验验证.
研究的目的:
- 为了研究寄生虫的自途径在素耐药性中的作用.
- 为了确定ER压力和未折叠蛋白反应 (UPR) 是否参与ART诱导的自.
- 建立自作为抗逆转录病毒耐药疟疾的治疗点.
主要方法:
- 对抗ART耐药性和野生型 (WT) *P.falciparum*分离物中自蛋白表达的比较分析.
- 通过饥饿和蛋白质水平的评估来诱导自.
- 使用自特异性抑制剂 (MRT68921) 进行药物敏感性测试.
- 在现场分离的*Pf*Kelch13和*Pf*ATG18突变的分析.
主要成果:
- 通过UPR通路,ART诱导的内等质网膜 (ER) 压力可以调节寄生虫自.
- 与WT相比,抗ART的Kelch13C580Y寄生虫表现出较高的基础和饥饿诱导的自蛋白表达.
- 自抑制显著降低了抗逆转录病毒耐药分离物中的寄生虫存活率,MRT68921.9的IC50较低.
- 在*Pf*Kelch13抗性突变 (C580Y,R539T,Y493H) 和*Pf*ATG18 (T38I) 突变之间存在强烈的关联.
结论:
- 自是一种关键的寄生虫存活机制在素抗性.
- 展开的蛋白质反应将ER压力与ART耐药性的自细胞升调联系在一起.
- 向自是一种有前途的策略,可以克服 *P. falciparum* 疟疾中的素抗性.
相关概念视频
Resistance
4.3K
When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
4.3K
Resistivity
3.3K
When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
3.3K
Radical Formation: Abstraction
3.3K
The electron of an atom can be abstracted from a compound by a relatively unstable radical to generate a new radical of relatively greater stability. For example, an initiator which forms radicals by homolysis can abstract a suitable species like a hydrogen atom or a halogen atom from a compound to generate a new radical. This ability of radicals to propagate by abstraction is a crucial feature of radical chain reactions.
Even though homolysis produces radicals, it is different from radical...
Even though homolysis produces radicals, it is different from radical...
3.3K
Ohm's Law
648
Resistors are fundamental components in electrical circuits, often manufactured from metallic alloys or carbon compounds. They model a material's ability to resist the flow of electric current, a characteristic that is crucial in controlling and regulating electrical power within a circuit.
This current-resisting behavior of resistors is governed by Ohm's law, which states that the voltage across a resistor is directly proportional to the current flowing through it.
This current-resisting behavior of resistors is governed by Ohm's law, which states that the voltage across a resistor is directly proportional to the current flowing through it.
648
Radical Formation: Elimination
1.6K
Another method of radical formation is the elimination process. It is the opposite of the addition route and is driven by the instability of the radical. For example, as depicted in Figure 1, dibenzoyl peroxide yields a pair of unstable radicals upon homolysis. Given its instability, this radical spontaneously undergoes elimination via a C–C bond cleavage to form a relatively more stable phenyl radical. The mechanism involves cleavage of the bond between the α and β positions...
1.6K
Persuasion Strategies
38.3K
Researchers have tested many persuasion strategies, including the foot-in-the door and the door-in-the-face techniques, in a variety of contexts. Ultimately, the principles are effective in selling products and changing people’s attitude, ideas, and behaviors (Cialdini & Goldstein, 2004).
38.3K

