Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K
Western Blotting01:15

Western Blotting

14.9K
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
14.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Cobalt and nickel ion synergy promotes gene amplification and stable metal resistance in <i>Shewanella oneidensis</i>.

Applied and environmental microbiology·2026
Same author

Development of binding and activity inhibition assays for the antibiotic resistance-associated protein PhoQ.

bioRxiv : the preprint server for biology·2026
Same author

Development of covalent inhibitors for bacterial histidine kinases.

Chemical communications (Cambridge, England)·2026
Same author

Structural and Dynamics Analyses of β-Lactam Inhibition of <i><i>Streptococcus pneumoniae</i></i> Penicillin-Binding Protein 1b (PBP1b) Guide Interrogation of Structure-Activity Relationships.

ACS chemical biology·2026
Same author

Evaluating the Link between Efflux Pump Expression and Motility Phenotypes in <i>Pseudomonas aeruginosa</i> Treated with Virulence Inhibitors.

ACS infectious diseases·2025
Same author

Challenges of Biological Complexity in the Study of Nanotoxicology.

Chemical research in toxicology·2025

相关实验视频

Updated: May 22, 2025

The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

8.9K

用基于活动的探针解码利素结合蛋白.

Erin E Carlson1,2,3,4, Nicholas Sparks1, Shivani Diwakar4

  • 1Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, United States.

Accounts of chemical research
|May 21, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了化学探针来研究细菌细胞壁构建酶,称为青素结合蛋白 (PBPs). 这些工具有助于了解细菌是如何适应的,并提供了针对抗生素耐药性的新策略.

更多相关视频

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

9.6K
Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

4.9K

相关实验视频

Last Updated: May 22, 2025

The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

8.9K
Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

9.6K
Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

4.9K

科学领域:

  • 微生物学 微生物学
  • 生物化学 生物化学
  • 化学生物学 化学生物学

背景情况:

  • 细菌细胞壁,主要是糖甘 (PG),对生存至关重要,也是关键的抗生素标.
  • 青素结合蛋白 (PBPs) 对于PG合成和重塑至关重要,但它们的调节和多种作用仍然不清楚.
  • 了解PBPs对于开发新抗生素来对抗耐药性至关重要.

研究的目的:

  • 开发选择性化学工具,特别是基于活动的探针 (ABP),以调查各种PBP同类的功能.
  • 探索PBPs在细菌细胞壁结构和适应环境压力的作用.
  • 识别用于准PBPs和克服抗生素耐药性的新型化学支架.

主要方法:

  • 在各种细菌物种中对β-乳酸胺抑制特征的系统评估.
  • 新型化学探针的设计和合成,包括选择性β-乳支架.
  • 应用这些化学工具来研究*Streptococcus pneumoniae*,*Escherichia coli*和*Bacillus subtilis*中的PG生物合成.

主要成果:

  • 确定了用于化学遗传研究的特定β-乳酸盐,并作为ABP开发的支架.
  • 发现了一种新的β-乳支架,对PBPs具有高选择性.
  • 获得了关于PG生物合成,细胞壁重塑和关键细菌病原体应激适应的新见解.

结论:

  • 选择性化学探针是剖析PBP功能和细菌细胞壁动态的强大工具.
  • 开发的探针和已识别的支架为新型抗生素发现提供了有前途的途径.
  • 对PBP多样性和监管的进一步研究对于应对抗菌素耐药性的挑战至关重要.