用IR Biotyper®系统对富里埃转换红外光谱的评估,用于Streptococcus pneumoniae血型鉴定
Camila Tondolo Romero1,2, Natália Kehl Moreira3,4, Gabriela Rosa da Cunha3,4
1Laboratório de Pesquisa Em Bacteriologia Clínica, LaBaC, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, Brazil. camila.tondolo@gmail.com.
概括
对于IR Biotyper®系统而言,一个新的定制分类器准确地测定了Streptococcus pneumoniae的血清型,为传统方法提供了更快的替代方案. 这种方法显示了关键血清型的性能改善,并区分了密切相关的菌株.
科学领域:
- 微生物学与传染病的研究
- 分析化学 分析化学
- 生物信息学和计算生物学
背景情况:
- 肺炎链球菌 (Streptococcus pneumoniae) 是人类的主要病原体,其囊对毒性至关重要.
- 精确的S. pneumoniae血清型定型对于临床和流行病学监测至关重要.
- 目前的黄金标准血清定型 (Quellung反应) 是耗时且资源密集的.
研究的目的:
- 评价IR Biotyper®系统,使用福里埃变换红外光谱 (FT-IRS) 作为S. pneumoniae血型鉴定工具.
- 开发和评估一个定制的分类器,专门针对肺炎球菌结合疫苗 (PCV15) 血清型.
主要方法:
- 商业Pneumococci血清分组 (Bruker) 的初步评估,其中有188个回顾性S. pneumoniae分离物.
- 在1246个频谱上使用人工神经网络 (ANN) 机器学习开发和验证PCV15特定分类器.
- 使用来自巴西南部的61个S. pneumoniae分离物的两种分类器的前性评估.
主要成果:
- 定制的PCV15-Classifier与商业血清组 (68.1%的回顾,70.5%的前) 相比,表现出更高的同意率 (86.7%的回顾,75.4%的前).
- PCV15-分类器在流行病学上显著的血清型中表现出卓越的表现,包括血清型3.
- 该分类器有效地区分了19血清组 (19F和19A) 内的密切相关的血清型.
结论:
- 通过定制分类器增强的IR Biotyper®系统是S. pneumoniae血型鉴定的一个有价值的工具.
- 这种基于FT-IRS的方法提供了比传统方法更及时,更具成本效益的替代方案.
- 分类器的重新培训能力使其能够适应不断变化的血清型流行率.
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