表面电荷在确定银纳米颗粒的细胞毒性,细胞吸收和生物分布方面取代了蛋白冠状形成
Marianna Barbalinardo1, Francesca Chiarini2, Gabriella Teti3
1Consiglio Nazionale delle Ricerche, Istituto per lo Studio dei Materiali Nanostrutturati (CNR-ISMN), via P. Gobetti 101, 40129 Bologna, Italy.
ACS applied bio materials
|May 21, 2025
概括
表面电荷,而不是蛋白质冠状,决定了银纳米粒子 (AgNP) 与细胞的相互作用. 带正电荷的AgNP显示出更高的细胞吸收和毒性,影响生物医学应用.
科学领域:
- 纳米医学是一种纳米医学.
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
背景情况:
- 银纳米粒子 (AgNPs) 在生物医学中表现有前途,但在临床转化方面面临挑战.
- 了解纳米-生物接口相互作用和蛋白质冠状病毒效应对于AgNP疗法至关重要.
研究的目的:
- 研究表面电荷和蛋白冠对AgNP细胞毒性,细胞吸收和生物分布的影响.
- 确定用于生物医学应用的AgNP细胞相互作用的主要因素.
主要方法:
- 负电荷和正电荷AgNP的比较.
- 分析蛋白冠状形成及其对AgNP细胞相互作用的影响.
- 在人类乳腺癌细胞中评估细胞毒性,细胞吸收和生物分布.
主要成果:
- 蛋白质冠状形成对AgNP可用性至关重要,但独立于表面电荷.
- 表面电荷显著影响AgNP细胞相互作用,正电荷增强吸收和毒性.
- 与负电荷的AgNP相比,负电荷的AgNP会导致更大的线粒体损伤和溶酶体积累.
结论:
- 表面电荷是AgNP细胞相互作用的主要因素,超过蛋白质冠冕效应.
- 冠状蛋白在生理环境中稳定AgNP起着至关重要的作用.
- 量身定制AgNP表面电荷对于优化其生物医学应用和安全概况至关重要.
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