三种转录因子形成了一个激活抑制模块,以调节亚洲杂交百合花中安东的积累
Mengmeng Bi1,2, Leifeng Xu1, Jiawen Wang1
1State Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Flower Crops, Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Plant physiology
|May 21, 2025
概括
亚洲杂交百合 亚洲杂交百合
科学领域:
- 植物分子生物学 植物分子生物学
- 花色素的遗传学 花色素的遗传学
背景情况:
- 安托素颜料决定了百合花的花色.
- 已知转录因子对安托西亚宁合成的调节,但HD-Zip蛋白和基因素乙化的作用尚不清楚.
研究的目的:
- 研究HD-Zip II家族蛋白质LhHB4在亚洲杂交百合花中调节氨酸积累中的作用.
- 为了阐明涉及LhHB4的分子机制,基因素乙化和安东生物合成.
主要方法:
- 鉴定LhHB4作为HD-Zip II家族成员.
- 对LhHB4与LhMYBSPLATTER和LhWRKY44促进体相互作用的分析.
- 研究LhHB4与LhTOPLESS相关蛋白 (LhTPR3) 的相互作用.
- 通过ChIP-qPCR评估素H3乙化水平.
主要成果:
- LhHB4 作为抑制安托素积累的抑制剂.
- LhHB4直接抑制LhMYBSPLATTER,并通过抑制LhWRKY44.4来间接抑制它.
- LhHB4与LhTPR3形成复合体,降低了基因素乙化和基因表达.
- 在LhHB4和LhMYBSPLATTER之间的反循环微调了花朵的色彩.
结论:
- LhHB4,LhWRKY44和LhMYBSPLATTER形成了一个调节模块,控制着氨酸生物合成.
- 希斯乙化在调节百合花中安东素水平方面发挥着至关重要的作用.
- 这项研究为花颜色的表观遗传调节提供了新的见解.
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