三重阴性乳腺癌中的原结构
Zoë Dinkel1, Adam Baker1, Alannah Akins1
1Department of Bioengineering, Clemson University, Clemson, South Carolina, United States of America.
PloS one
|May 21, 2025
概括
与白人患者相比,患有三阴性乳腺癌 (TNBC) 的非裔美国患者表现出明显的原基质差异. 这些宏观原蛋白变异可能表明非洲裔美国人TNBC病例中的瘤表型更具侵略性.
科学领域:
- 在瘤学瘤学.
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
背景情况:
- 三阴性乳腺癌 (TNBC) 不成比例地影响非裔美国 (AA) 女性,与较高的死亡率相关.
- 瘤微环境富含细胞外基因原,在癌症的进展,转移和治疗耐药性方面发挥着至关重要的作用.
- 增加的原沉积,与密集的乳腺组织相关,在AA妇女中被假设,并与更具侵略性的癌症有关.
研究的目的:
- 调查来自非洲裔美国人和白人患者的TNBC样本之间的纤维状原蛋白结构差异.
- 确定是否存在宏观结构或微观结构的原蛋白变异,并与种族群体相关联.
主要方法:
- 利用带有第二子生成 (SHG) 的双光子显微镜可视化TNBC组织样本中的纤维状原体.
- 分析了每个样本的20个区域,使用斐济宏观TWOMBLI进行了对中镜和纳米镜原蛋白性质的定量评估.
- 量化参数,如纤维面积,总长度,端点,分支点和缺口,以及纤维的排列和包装密度.
主要成果:
- 与高加索样本相比,AA患者的TNBC样本显示了显著增加的美索斯科普原参数,包括纤维面积,总长度和终点和分支点的数量.
- 从AA样本的原体中观察到缺口性显著减少,表明纤维组织发生了变化.
- 在种族群体之间,在原体微观结构上没有发现显著差异,例如纤维的排列和包装密度.
结论:
- 来自AA患者的TNBC样本中的宏观原蛋白特征与高加索患者的特征有显著差异.
- 这些观察到的原蛋白差异可能与非洲裔美国人TNBC中更具侵略性的瘤表型有关.
- 需要进一步的研究,以了解这些种族差异在原结构中的临床影响.
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