在UV-C辐射下对乳制品菌体无活化的动态建模
María Fiorella Jacob1, Andrea Del Luján Quiberoni1, María de Los Milagros Ballari2
1Instituto de Lactología Industrial (Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas), Facultad de Ingeniería Química, Santiago del Estero 2829, 3000 Santa Fe, Argentina.
The Science of the total environment
|May 21, 2025
概括
紫外线 (UV-C) 辐射有效地使干燥表面上的乳制品菌体失活,这对工业卫生至关重要. 动力模型预测了无活化,有助于制定食品加工环境的有效消毒指南.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 工程 工程师 工程师 工程师
背景情况:
- 乳制品菌体在工业环境中构成重大挑战,有助于通过生物气溶和受污染的表面传播菌体.
- 之前的研究主要集中在液体悬浮中的菌体无活化上,这在了解工业环境相关的干表面无活化方面存在差距.
- 许多乳制品菌体,特别是特定地区的特有菌体,在对UV-C消毒技术的敏感性方面仍然没有特征.
研究的目的:
- 评估近紫外线辐射 (λ = 254 nm) 在干燥表面无活化十六种乳制品菌体的有效性.
- 调查菌体无活化动力学,并确定适合预测无活化的模型.
- 为乳制品行业提供数据支持UV-C消毒策略的开发.
主要方法:
- 乳制品菌体悬浮物 (106-107 PFU/mL) 被干燥在玻璃板上.
- 干燥的菌体样本暴露于日益增加的紫外线-C辐射剂量 (λ = 254 nm).
- 评估了受治疗的菌体的传染性,并使用八种不同的模型建模了无活化动力学.
主要成果:
- 在16个乳制品菌体中,有6个在0.13J/cm2的UV-C剂量下完全失活.
- 其余的十种菌体在0.13或0.32J/cm2的剂量下显示了从3.16到4.45日志序的标位降低.
- 在较高剂量下观察到十种菌体的尾巴效应,而Geeraerd和Baranyi和Roberts模型最好地预测了无活化动力学.
结论:
- 紫外线辐射是一种可行且具有成本效益的方法,用于在工业环境中的干燥表面禁用乳制品菌体.
- 已识别的UV-C剂量适用于工业应用,需要最小的设备和维护.
- 精确的动力模型可以预测菌体不活化,支持优化食品加工环境中的UV-C消毒协议.
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