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针对CD177⁺中性粒细胞的线粒体复合体I减轻了肺缺血-再输液损伤

Junqi Wu¹, Peigen Gao¹, Chenlu Yang²

¹Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China; Shanghai Engineering Research Center of Lung Transplantation, Shanghai, China.

Cell reports. Medicine

|May 21, 2025

概括

激活的CD177+中性粒细胞通过增加炎症和线粒体活动来驱动肺移植损伤. 准线粒体功能为初级移植功能障碍提供了潜在的治疗方法.

科学领域:

免疫学免疫学免疫学
移植生物学移植生物学
细胞生物学细胞生物学

背景情况:

主要移植功能障碍 (PGD) 是肺移植后的主要死亡原因.
中性粒细胞是PGD炎症过程中的关键参与者.

研究的目的:

为了研究参与肺缺血-再输液损伤 (IRI) 的中性粒细胞亚型.
确定PGD的潜在生物标志物和治疗点.

主要方法:

在小鼠IRI模型中使用了单细胞RNA测序和空间转录组学.
人类肺移植接受者被分析了潜在的生物标志物.
在小鼠和老鼠模型中使用IACS-010759准线粒体功能.

主要成果:

鉴定出CD177+中性粒细胞是活性亚种群,对肺损伤有显著的贡献.
CD177+中性粒细胞显示氧化酸化和线粒体复合体I活性增加.
在人类接受者中,CD177+中性粒细胞预测了严重的PGD (AUC = 0.871).
针对线粒体复合体I减少了CD177+中性粒细胞的激活,并减轻了肺损伤.

结论:

CD177+中性粒细胞是肺IRI和PGD的关键驱动因素.
CD177+中性粒细胞作为严重PGD的早期诊断生物标志物.
抑制线粒体功能是PGD的一个有前途的治疗策略.

关键词:

CD177 是一个CD177 是一个CD177 肺缺血-再输液损伤的肺缺血.线粒体复合体I 线粒体复合体I 作为一个中性恋者.氧化酸化是一种氧化酸化.

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