高分辨率测序显示,Paf1复合体可能是真核细胞RNA聚合酶I的保存转录延长因子
Abigail K Huffines1, Naiheng J Yang1, David A Schneider1
1Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294.
Journal of molecular biology
|May 21, 2025
概括
聚合酶相关因子1复合体 (Paf1C) 对于RNA聚合酶I (Pol I) 转录至关重要. 丢失Paf1C子单元减少了基因5'末端的Pol I占用,这表明在核糖体RNA合成中保留了作用.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 单核细胞转录 单核细胞转录
背景情况:
- 细胞利用三种RNA聚合酶 (Pol I,II,III) 来进行不同的RNA合成.
- 转录效率受到各种转录作用因子的调节,其中一些影响多个聚合酶.
- 聚合酶相关因子1复合体 (Paf1C) 是一种已知影响Pol I和Pol II活性的保存转录因子.
研究的目的:
- 研究Paf1C在RNA聚合酶I (Pol I) 转录中的作用.
- 为了确定Paf1C子单位损失对单核酸分辨率下的Pol I占用量的影响.
- 探索Paf1C在跨物种的核糖体RNA合成中的保存功能.
主要方法:
- 在Saccharomyces cerevisiae (酵母菌) 突变体 (paf1Δ和cdc73Δ) 中进行原生延伸转录测序 (NET-seq).
- 分析PRO-seq数据从DLD1哺乳动物细胞与Paf1C淘汰.
- 对Pol I占用模式的单核酸分辨率分析.
主要成果:
- 酵母中Paf1C子单元 (Paf1,Cdc73) 的损失导致DNA模板的5'端的Pol I占用率显著降低.
- 在 paf1Δ 和 cdc73Δ 突变中观察到整个基因的 Pol I 占用模式的变化.
- 哺乳动物细胞中的Paf1C敲击也显示了5'端的Pol I占用率降低,与酵母发现一致.
结论:
- Paf1C是Pol I.的一个重要的转录延长因子.
- Paf1C在调节真核生物中的Pol I转录中起着保守的作用.
- 这些发现强调了Paf1C在核糖体RNA合成中的重要性.
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