废水监测以估计和描述肝炎E病毒的循环
C Dimeglio1,2, O Schlosser3, S Laperche4
1Laboratoire de Virologie, Centre National de Référence Virus de l'Hépatite E, CHU Toulouse-Purpan, 31059, Toulouse, France. dimeglio.c@chu-toulouse.fr.
Food and environmental virology
|May 21, 2025
概括
废水监测有效地追踪了法国城市的肝炎E病毒 (HEV) 循环和多样性. 这种方法为HEV流行病学和人口感染状况提供了宝贵的见解.
科学领域:
- 环境病毒学环境病毒学
- 公共卫生监督是对公共卫生的监督.
- 分子流行病学分子流行病学
背景情况:
- 肝炎E病毒 (HEV) 在全球引起肠道传播的肝炎.
- 无症状的HEV感染很常见,导致其真正的流行率被低估.
- 废水监测提供了一个潜在的工具,以更好地了解HEV流行病学.
研究的目的:
- 评估废水监测对HEV循环和多样性的监测能力.
- 为了比较法国图卢兹和敦刻尔克的HEV流行病学.
- 为了将废水病毒载荷与服务人口中的HEV感染相关联.
主要方法:
- 在废水中使用数字PCR检测和定量HEVRNA.
- 通过长读单分子实时测序进行HEV基因型定型.
- 在使用商业测试的献血者中检测HEV RNA.
主要成果:
- 在88% (图卢兹) 和95% (敦刻尔克) 的废水样本中检测到HEV RNA.
- 确定了HEV-3亚型 (HEV-3c,HEV-3f,HEV-3h),其中HEV-3c在两个城市都占主导地位.
- 废水HEVRNA峰值与图卢兹的献血者感染相关 (3周的滞后),但在克尔克的感染程度较低.
结论:
- 污水监测是评估当地HEV感染状况和流通的宝贵工具.
- 该研究强调了废水监测对于了解HEV流行病学和多样性的有用性.
- 当地废水监测系统可以为公共卫生干预提供关键数据.
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