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相关概念视频

Evolutionary Relationships through Genome Comparisons02:54

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Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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相关实验视频

Updated: May 23, 2025

BEST: Barcode Enabled Sequencing of Tetrads
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一个多王国遗传条形码系统,用于精确的克隆隔离.

Soh Ishiguro1, Kana Ishida2, Rina C Sakata1

  • 1School of Biomedical Engineering, Faculty of Applied Science and Faculty of Medicine, The University of British Columbia, Vancouver, British Columbia, Canada.

Nature biotechnology
|May 21, 2025
PubMed
概括
此摘要是机器生成的。

CloneSelect使用DNA条形码和CRISPR基编辑来从复杂群体中分离特定的细胞克隆. 这种遗传条形码系统可以为各种研究应用进行有针对性的细胞隔离.

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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
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相关实验视频

Last Updated: May 23, 2025

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

10.0K
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物技术是生物技术.

背景情况:

  • DNA条形编码有助于分析细胞克隆动态和转录组学.
  • 从异质细胞群中分离特定的表型是一个重大挑战.

研究的目的:

  • 开发一种多功能基因条码系统,以根据表型分离目标细胞克隆.
  • 通过CRISPR基基编辑来实现针对性记者基因表达以进行克隆分离.

主要方法:

  • 开发了克隆Select,一个多王国遗传条形码系统.
  • 利用条形码特定的CRISPR基编辑来触发目标克隆中的记者基因表达.
  • 与单细胞RNA测序集成,用于全面分析.

主要成果:

  • 在各种细胞类型中成功分离了目标克隆,包括人类和小鼠细胞,酵母和细菌.
  • CloneSelect被证明是可扩展的,并且与下游分析 (如单细胞RNA测序) 兼容.
  • 能够从复杂的混合物中分离出具有特定表型的克隆.

结论:

  • CloneSelect提供了一种强大而通用的方法,可以在多个王国中分离特定的细胞克隆.
  • 这项技术推动了细胞异质性研究和以表型为驱动的研究.
  • 便于精确操纵和分析各种生物系统中的细胞群.