在IPEC-J2细胞中,Parabacteroides goldsteinii减轻了脂多糖诱导的炎症反应
Junjie Zhang1, Anjian Li1, Yang He1
1Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.
Frontiers in veterinary science
|May 22, 2025
概括
黄金杆菌 (PG) 显示出作为动物养殖中抗生素的益生菌替代品的希望. 这项研究发现,PG有效减少炎症并改善小猪的肠道健康.
科学领域:
- 动物科学动物科学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 在畜牧业中滥用抗生素对动物健康,食品安全和农业发展构成风险.
- 益生菌在畜牧业和家禽养殖中为抗生素提供了有效和环保的替代品.
- *Parabacteroides goldsteinii* (PG) 的抗炎作用已在小鼠中观察到,但其对其他动物的影响尚未得到充分证实.
研究的目的:
- 研究 *Parabacteroides goldsteinii* (PG) 作为益生菌改善小猪肠道健康的疗效.
- 评估PG对猪肠上皮细胞中脂聚糖 (LPS) 诱导的炎症的抗炎作用 (IPEC-J2).
- 通过转录组分析分析PG的影响背后的分子机制.
主要方法:
- 在IPEC-J2细胞中使用PG超和脂聚糖 (LPS) 诱导炎症.
- 测量了促炎和抗炎细胞因子水平 (IL-6,IL-8,IL-10).
- 进行了转录组分析 (RNA测序) 以识别差异表达基因 (DEG) 并通过基因本体学 (GO) 和基因和基因组 (KEGG) 基因百科全书 (Kyoto Encyclopedia of Genes and Genomes) 路径丰富分析分析它们的功能.
主要成果:
- 在20倍稀释时,PG超剂显示出最佳的细胞活力.
- 通过减少促炎性细胞因子IL-6和增加抗炎性细胞因子IL-10,PG显著减轻了LPS诱导的炎症.
- 转录组分析显示,LPS和LPS-PG组之间有2,126个差异表达的基因,其中关键基因与炎症相关 (例如IL-6R,NF-kB) 以及包括PI3K-AKT信号传递在内的丰富通路.
结论:
- *Parabacteroides goldsteinii* 具有显著的抗炎特性,并且有效地减轻猪肠细胞中LPS诱导的炎症反应.
- *P. goldsteinii*积极调节与炎症和免疫路径相关的基因表达.
- 这些发现支持P. goldsteinii作为强大的益生菌候选人,可以改善小猪的肠道健康和功能.
关键词:
在IPEC-J2单元中,单元是IPEC-J2单元.通过PI3K-Akt路径.帕拉巴克特罗伊德斯金石种子抗炎机制的抗炎机制.脂多糖类糖化物 (Lipopolysaccharide) 是一种脂多糖类糖.更多相关视频
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