相关实验视频

Updated: Jul 28, 2026

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

离子凝振动喷流技术的多响应表面优化,以微调从麦中提取的咖啡因微粒干蒸机的谷物

Umar Shah¹, Rewati R Bhattarai¹, Hani Al Salami²

¹School of Molecular and Life Sciences, Faculty of Science and Engineering, Curtin University, Perth, Western Australia, Australia.

Journal of food science

|May 22, 2025

概括

干蒸机的谷物与可溶性 (DDGS) 卡菲林蛋白被净化并使用离子凝振动喷射流技术形成微粒. 这种可持续的方法可以为生物材料应用产生统一的微粒.

科学领域:

生物材料科学生物材料科学
蛋白质化学蛋白质化学
化学工程是化学工程的重要组成部分.

背景情况:

干蒸机含可溶性谷物 (DDGS) 是生物乙醇生产中富含蛋白质的副产品,通常被作为废物丢弃.
在DDGS中含有的胺蛋白卡菲林具有独特的特性,如疏水性和二硫化物交叉链接,这表明生物材料开发的潜力.
目前基于蛋白质的微粒生产方法在可扩展性和统一性方面存在局限性.

研究的目的:

为了从 Sorghum DDGS 中净化卡菲林蛋白.
开发和优化一种可扩展的方法,使用离子凝振动喷流技术 (IGVJFT) 生产卡菲林微粒.
描述产生的微粒的物理化学性质,并确定最佳加工条件.

主要方法:

咖啡因蛋白从 DDGS 中提取,使用乙醇提取和酸沉.
使用离子凝振动喷流技术 (IGVJFT) 制造了微粒.
使用中央复合设计的响应表面方法 (RSM) 来优化IGVJFT参数 (电压,频率,蛋白质度) 并评估微粒子特征 (大小,泽塔潜力,断裂频率).

主要成果:

纯化卡菲林蛋白被成功地用于制造微粒.
通过IGVJFT,可以生产具有可调节性质的均球形卡菲林微粒.
在优化条件下,微粒的尺寸从406.7到656.4微米,泽塔电位从-38.2到-18.1mV,机械强度 (断裂频率) 达到23%-57%.

结论:

DDGS卡菲林是微粒制造的可行和可持续的蛋白质来源.
IGVJFT是一种有效且可扩展的技术,用于生产具有受控物理化学性质的疏水性蛋白质基微粒.
这项研究证明了DDGS卡菲林微粒在各种大型生物材料应用中的潜力.

关键词:

在DDGS中,Kafirin是最重要的.离子凝振动喷射流技术的技术这些微粒是微粒.响应表面方法的方法.

更多相关视频

Reactive Inkjet Printing and Propulsion Analysis of Silk-based Self-propelled Micro-stirrers

Reactive Inkjet Printing and Propulsion Analysis of Silk-based Self-propelled Micro-stirrers

Published on: April 26, 2019

Author Spotlight: Advancing Therapeutics with Biocompatible Sodium Alginate Hydrogel Microspheres

Author Spotlight: Advancing Therapeutics with Biocompatible Sodium Alginate Hydrogel Microspheres

Published on: June 7, 2024

相关概念视频

Bioreactor Controls-II

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

Methods of Medium Optimization

Methods of Medium Optimization

Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...