相关实验视频

Updated: May 23, 2025

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

Published on: February 23, 2014

干细胞CNTF促进嗅觉上皮质神经再生和受伤后的功能恢复

Derek Cox¹, Brian Wang¹, Joe Oliver¹

¹Department of Biomedical Sciences, Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, United States of America.

Stem cells (Dayton, Ohio)

|May 22, 2025

概括

纤毛神经变因子 (CNTF) 促进了炎症后嗅觉神经元 (OSN) 的再生. 这项研究表明,CNTF增强了基底干细胞的增殖,有助于嗅觉功能的恢复.

科学领域:

神经科学是一个神经科学.
再生医学是一种再生医学.
耳鼻喉科耳鼻喉科耳鼻喉科

背景情况:

嗅觉感官神经元 (OSNs) 从嗅觉上皮质 (OE) 中的基底干细胞再生.
急性炎症可以破坏OSNs,导致嗅觉功能障碍,但基底干细胞仍然可活.
纤毛神经变因子 (CNTF) 表达在水平基底细胞 (HBCs) 中,其受体在全球基底细胞 (GBCs) 中,它们是活跃分裂的原始细胞.

研究的目的:

研究CNTF在基底干细胞增殖和分化中的作用.
确定CNTF对经过OE炎症损伤后嗅觉功能的恢复的影响.
阐明针对嗅觉再生的CNTF信号的治疗潜力.

主要方法:

在小鼠中使用甲基马诱导的急性炎症性OE损伤.
评估了炎症标志物 (TNFα,IL-6,CD45) 和 OSN 的减少.
在CNTF+/+和CNTF-/-小鼠中使用BrdU标签量化GBC增殖.
评估了嗅觉功能的恢复和神经再生水平.

主要成果:

甲马诱导炎症和OSN损失,同时上调CNTF和GBC标志物Mash1.
作为对损伤的反应,CNTF显著促进了GBC的扩散.
与CNTF-/-小鼠相比,CNTF+/+小鼠表现出增强的神经再生和优异的嗅觉功能恢复.
初级HBC培养证实了HBC产生的CNTF,以刺激GBC的增殖.

结论:

在嗅觉神经再生过程中,CNTF在促进全球基底细胞增殖方面发挥着至关重要的作用.
在炎症性OE损伤后,CNTF信号对于恢复嗅觉功能至关重要.
向CNTF提供了一个有前途的治疗策略,以增强嗅觉再生和功能恢复.

关键词:

埋藏的食物测试试验全球球体基底细胞基底细胞横向的基本细胞水平细胞.这是一种炎症炎症炎症炎症.嗅觉习惯/不习惯测试嗅觉感官神经元的神经元

更多相关视频

Isolating Nasal Olfactory Stem Cells from Rodents or Humans

Isolating Nasal Olfactory Stem Cells from Rodents or Humans

Published on: August 22, 2011

Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration

Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration

Published on: November 2, 2020

相关概念视频

Neurogenesis and Regeneration of Nervous Tissue

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...