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一个用于神经科学研究的ATAC-seq协议

Nur Hasan Prince¹, Misako Hamamura¹, Yi Zhou^2,3

¹Department of Anatomy and Developmental Biology, School of Medicine, Shimane University, 89-1, En-Ya-Cho, Izumo-Shi, Shimane, 693-8501, Japan.

Anatomical science international

|May 22, 2025

概括

这项研究优化了通过神经组织的测序 (ATAC-seq) 优化转移酶可访问染色质的测定. 增强的协议允许使用最小的细胞输入进行敏感的神经表观遗传学分析.

科学领域:

神经科学是一个神经科学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

染色体可访问性映射对于理解神经科学中的基因调节至关重要.
现有的ATAC-seq协议面临着敏感的神经组织的挑战.

研究的目的:

为神经组织和细胞提供一个针对神经组织和细胞的优化ATAC-seq协议.
解决神经样本中保护色素完整性的挑战.

主要方法:

神经组织处理和核隔离的详细协议.
优化转换反应和图书馆准备用于神经ATAC-seq.
这种方法对最小的起始材料 (20,000-50,000 个细胞) 进行了验证.

主要成果:

在神经样本中对色素可访问性的高分辨率映射.
在神经发育和神经障碍中敏感检测调节元件.
强大的神经表观遗传学数据生成.

结论:

优化的ATAC-seq协议对神经应用有效.
有助于对神经表观遗传学调节进行强有力的研究.
能够研究神经可塑性和神经疾病.

关键词:

ATAC-seqq 的使用情况.脑子脑子脑子脑子染色是一种染色素.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.神经解剖学是一个神经解剖学.

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