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Updated: Jul 15, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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系统地调查CRISPR/Cas12a光生物传感器用于敏感和特定的单核酸变体检测.

Zhenlin Shang1, Sitong Liu1, Dongxu Liu1

  • 1School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing, 100048, P. R. China.

Journal of fluorescence
|May 22, 2025
PubMed
概括

这项研究开发了一个CRISPR/Cas12a系统,具有优化的crRNA和分子信标记者,用于精确检测单核酸变异 (SNV). 该方法准确地识别了SARS-CoV-2的突变,如D614G和N501Y,从而可以快速检测病原体.

关键词:
这就是CRISPR/Cas12a.D614G 是一个D614G.N501YY 这是一个很好的例子.单核酸变异的变化

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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...