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Updated: Jun 13, 2025

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使用基于人类多能干细胞的有机分化器官进行早期胰腺发育的特异性和形态发生在体外建模的协议

Chenglei Tian1, Ulf Tiemann2, Florian Hermann2

  • 1Institute of Translational Stem Cell Research, Helmholtz Diabetes Center, Helmholtz Zentrum Munchen, 85764 Munich, Germany; Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), University of Copenhagen, 2200 Copenhagen, Denmark.

STAR protocols
|May 22, 2025
PubMed
概括

这项研究详细介绍了一种使用Matrigel覆盖器官分化的人类多能干细胞 (hPSCs) 来产生胰腺细胞的协议. 该方法可以通过创建关键的胰腺细胞类型来进行体外器官发育和疾病的研究.

关键词:
细胞生物学 细胞生物学细胞分化 细胞分化细胞培养培养的细胞培养发育生物学是发展生物学.器官类动物 器官类动物干细胞是干细胞.

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The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
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