2型细胞因子对肠道感官神经元起作用,调节神经驱动的主体防御
Rocky M Barilla1,2, Clara Berard1, Linyu Sun1
1Gene Lay Institute of Immunology and Inflammation, Brigham and Women's Hospital, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
概括
2型细胞因子在肠道神经元中增强神经胺U (NMU) 和与素基因相关的β (CGRPβ),增强宿主对虫感染的防御能力. 这突显了肠道中神经免疫系统的重要沟通.
科学领域:
- 神经免疫学
- 胃肠病学
- 传染性疾病
背景情况:
- 肠道神经系统 (ENS) 神经会影响免疫细胞,但炎症在感染期间如何影响肠道神经元尚不清楚.
- 产生神经胺U (NMU) 和与素基因相关的β (CGRPβ) 的初级肠道感觉神经元 (PSN) 共同表达2型细胞因子受体 (IL-4,IL-13).
研究的目的:
- 研究2型细胞因子如何调节PSN中的NMU和CGRPβ表达.
- 确定IL-13受体子单元α (IL-13RA1) 在虫感染期间的PSN反应和宿主防御中的作用.
主要方法:
- 表示NMU和CGRPβ的PSN子集的特征.
- 关于2型细胞因子对神经表达的体外和体内研究.
- 分析PSN特异性Il13ra1删除的小鼠在Heligmosomoides polygyrus感染期间的宿主防御和免疫反应
- 评估NMU23和CGRPβ同时使用的治疗效果.
主要成果:
- 2型细胞因子显著增加了PSN中的NMU和CGRPβ表达.
- 特定于PSN的Il13ra1删除取消了细胞因子诱导的神经的表达.
- 在PSN中缺失Il13ra1导致虫清除和肌肉免疫反应减弱.
- 同时使用NMU23和CGRPβ恢复了抗虫免疫力.
结论:
- 2型细胞因子通过IL-13RA1直接增强肠道感官神经元中的NMU和CGRPβ表达.
- 涉及NMU和CGRPβ的双向神经免疫交叉对话对于调节2型肠道炎症和对虫的宿主防御至关重要.
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