糖酸脱酶是内核发育所需的,并定义了玉米中占主导地位的氨酸合成途径
Ying Zhang1, Ruoxuan Li2, Daocan Zheng3
1The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education; Shandong Key Laboratory of Precision Molecular Crop Design and Breeding; State Key Laboratory of Microbial Technology; School of Life Sciences, Shandong University, Qingdao 266237, China.
The Plant cell
|May 22, 2025
概括
玉米基因Dek20调节了血清蛋白生物合成. 一种突变破坏了这一点,降低了血清水平并影响了翻译,揭示了C4植物中的营养感应机制.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 氨酸对于植物中的蛋白质合成和信号传递至关重要.
- 调节血清蛋白生物合成及其下游效应的精确机制尚未完全理解.
研究的目的:
- 为了克隆和表征玉米 (Zea mays) 的基因Dek20,编码糖酸脱酶1 (PGDH1).
- 阐明Dek20在血清蛋白生物合成和营养感应中的作用.
主要方法:
- 基因克隆和 Dek20 的功能表征.
- 分析 dek20(Ser282Leu) 突变对酶活性和蛋白质相互作用的影响.
- 转录组分析,多元体分析和Ribo-seq来评估翻译效率.
主要成果:
- 德克20突变破坏了PGDH1的功能,抑制了血清蛋白生物合成.
- 血清素缺乏导致tRNASer降解和停滞的核糖体.
- 停滞的核糖体激活GCN2激酶,抑制翻译启动和延长.
- 翻译效率降低会影响储存化合物和细胞循环至关重要的蛋白质.
结论:
- 德克20是玉米酸化途径的氨酸生物合成 (PPSB) 中的主要酶.
- 这项研究揭示了在玉米中氨基酸稳定和营养感应的新机制.
- 研究结果提供了关于C4植物对氨基酸缺乏症的转化调节的见解.
相关概念视频
Glycolysis: Preparatory Phase
13.3K
In cellular metabolism (the complete breakdown of glucose to extract energy), glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
13.3K
Glycolysis: Pay-off Phase
9.8K
So far, glycolysis has cost the cell two ATP molecules and produced two small, three-carbon sugar molecules. These molecules will proceed through the second half of the pathway, and sufficient energy will be extracted to pay back the two ATP molecules used as an initial investment and produce a profit for the cell of two additional ATP molecules and two even higher-energy NADH molecules.
Step 1 - 5: Glycolysis Preparatory Phase
The first phase of glycolysis has 5 steps where the glucose is...
Step 1 - 5: Glycolysis Preparatory Phase
The first phase of glycolysis has 5 steps where the glucose is...
9.8K
Protein Kinases and Phosphatases
13.1K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
13.1K
C4 Pathway and CAM
45.4K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.4K
Energy-requiring Steps of Glycolysis
163.3K
Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
163.3K
Energy-releasing Steps of Glycolysis
138.9K
Glycolysis is divided into two phases based on whether energy is utilized or released. While the first phase consumes ATP, the second phase produces energy in the form of ATP and NADH. The energy is released over a sequence of reactions that turns G3P into pyruvate. The energy-releasing phase—steps 6-10 of glycolysis—occurs twice, once for each of the two 3-carbon sugars produced during steps 1-5 of the first phase.
The first energy-releasing step—the 6th step of glycolysis...
The first energy-releasing step—the 6th step of glycolysis...
138.9K


