定数感应:在培养人类前兆细胞白血病细胞系HL-60及其与中性粒细胞相关的功能中的一个重要因素
Danfeng Li1, Tian Yang1, Siyuan Ma1
1Department of Orthodontics, Hospital of Stomatology, Sun Yat-sen University, Guangzhou 510055, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510080, China; Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510080, China.
Methods (San Diego, Calif.)
|May 22, 2025
概括
优化HL-60细胞培养密度可以增强中性粒细胞的增殖和抗菌活性. 增加的密度会促进分化,反应性氧物种的产生,以及通过细胞质感知通过细胞外囊泡介导的细胞化.
科学领域:
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
背景情况:
- HL-60细胞是中性粒细胞研究的标准体外模型.
- 培养HL-60细胞存在挑战,细胞培养密度在历史上被忽视.
- 优化培养条件对于一致和有效的HL-60细胞研究至关重要.
研究的目的:
- 为了优化HL-60细胞培养协议.
- 研究细胞培养密度对HL-60细胞增殖和分化的影响.
- 探索细胞密度对分化HL-60 (dHL-60) 中性粒细胞抗菌潜力的影响.
主要方法:
- 优化HL-60细胞培养协议,重点关注密度.
- 评估了细胞增殖,分化速率,线粒体反应性氧物种 (ROS) 生产和细菌细胞.
- 研究了小细胞外囊泡 (sEVs) 在HL-60细胞通信和增殖中的作用.
主要成果:
- 细胞增殖活动与细胞密度增加正相关.
- 较高的细胞密度导致分化的速度增加,ROS的产生,以及细菌的细胞化.
- 由HL-60衍生的sEVs (hEVs) 在低密度培养中恢复了繁殖,这表明了定数感应机制.
结论:
- 优化的培养密度显著提高HL-60细胞的增殖和抗菌活性.
- 细胞密度和sEV在中性粒细胞功能和通信中起着关键作用.
- 这些发现为体外HL-60细胞实验提供了重要的考虑因素,并表明中性粒细胞的定数检测.
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