相关实验视频

Updated: Sep 20, 2025

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

微RNA-196a通过降低FOXO1的调节来增加B细胞中的细胞灭亡

Soyoung Kim¹, Mina Han¹, Hyun Ju Hwang¹

¹Asan Institute for Life Sciences and Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.

Molecules and cells

|May 22, 2025

概括

微RNA-196a-5p (miR-196a-5p) 通过向FOXO1.1来调节B细胞恶性瘤. 抑制miR-196a-5p会增加B细胞的亡,这表明它在B细胞癌症中的治疗潜力.

科学领域:

分子生物学分子生物学
癌症研究癌症研究
免疫学免疫学免疫学

背景情况:

微RNAs (miRNAs) 在癌症发育中至关重要,癌细胞中常见的改变表达.
虽然miR-196a-5p在某些癌症中的作用已知,但其在B细胞恶性瘤中的功能仍未得到充分探索.
了解miRNA调节对于开发向癌症疗法至关重要.

研究的目的:

研究miR-196a-5p在B细胞中的作用和调控机制.
为了确定miR-196a-5p是否影响B细胞淋巴瘤细胞系和初级B淋巴细胞.
在B细胞激活和恶性瘤的背景下,确定miR-196a-5p的下游目标.

主要方法:

利用人类扩散大B细胞淋巴瘤细胞系 (SU-DHL-6) 和小鼠B淋巴细胞.
在SU-DHL-6细胞中执行了miR-196a的强制表达,以评估细胞亡.
进行了光酶检测,以确定miR-196a-5p的直接标.
分析了目标基因的mRNA和蛋白质表达水平.
研究了不同B细胞群中的miR-196a-5p表达及其与B细胞受体刺激的相关性.

主要成果:

强迫的miR-196a表达增强了SU-DHL-6细胞中的鲁素诱导的亡.
FOXO1被确定为miR-196a-5p的直接标,其表达在miR-196a-5p过度表达时被调低.
与脏B细胞相比,miR-196a-5p在小鼠骨髓B细胞中表达高,与FOXO1水平呈反相关性.
在B细胞受体刺激时,miR-196a-5p的表达增加,与FOXO1.1相反相关.
在初级小鼠B细胞中,miR-196a-5p的升级导致了亡的增加.

结论:

miR-196a-5p作为FOXO1.1的转录后调节剂.
这些发现突出了miR-196a-5p在调节B细胞恶性瘤中的重要作用.
miR-196a-5p与B细胞的激活和亡有关,这表明它有可能成为治疗点.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.B细胞激活激活的过程在B细胞淋巴瘤.这就是FOXO1的意义.微型RNA-196a-5p是什么意思

更多相关视频

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

相关概念视频

The Intrinsic Apoptotic Pathway

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

The Extrinsic Apoptotic Pathway

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...

Apoptosis

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...

Receptor Downregulation in MVBs

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...