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Updated: Sep 20, 2025

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研究工艺参数对单链可变碎片包容体的性能和再折叠产量的影响

Mohamed Elshazly¹, Benedikt Leeb¹, Eva Prada Brichtova¹

¹Research Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorferstraße 1A, Vienna 1060, Austria; Christian Doppler Laboratory for Inclusion Body Processing 4.0, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorferstraße 1A, Vienna 1060, Austria.

Journal of biotechnology

|May 22, 2025

概括

上游加工条件不会显著影响 scFvM 蛋白质的纳入体 (IB) 特性或重新折叠产量. 然而,优化这些条件可以增加IB标位,提高体积生产率.

科学领域:

生物技术是生物技术.
蛋白质表达方式
生物工艺工程生物工艺工程

背景情况:

包括体 (IB) 是用于生物制药生产的蛋白质聚合物.
了解上游加工 (USP) 对IB特性和重新折叠的影响对于高效的开发至关重要.
特定的USP参数对IB特性和重新折叠性能的影响仍然定义不佳.

研究的目的:

研究特定基质吸收率和感应温度如何影响IB标位,纯度,大小,二次结构和单链可变片段M (scFvM) 的重新折叠产量.
确定USP条件是否影响IB属性和scFvM的重新折叠效率.
确定最佳的USP战略,以最大限度地提高IB产量.

主要方法:

研究了特定基质吸收率和诱导温度对scFvM纳入体的影响.
分析IB标位,纯度,大小和二次蛋白质结构.
在不同的USP条件下,scFvM纳入体的评估重新折叠收益率.

主要成果:

上游加工条件没有显著改变IB纯度,尺寸或二次结构.
scFvM纳入体的重新折叠产量独立于测试的USP参数.
特定的基质吸收率和诱导温度显著影响IB标位,从而提高了体积生产率.

结论:

对于scFvM,IB特性和重新折叠性能在很大程度上独立于研究的USP参数.
通过USP变化最大化IB标位是提高体积生产力的可行策略.
这些发现可以指导其他具有类似结构特征的蛋白质的优化策略.

关键词:

包容性机构是包容性的机构.包括体的属性身体的属性综合生物工艺综合生物工艺多变量数据分析多变量数据分析.二次蛋白质结构二次蛋白质结构上游过程参数上游过程参数

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