BAF60/SWP73子单位定义了Arabidopsis中SWI/SNF染色体重塑复合物的子类
Sebastian P Sacharowski1, Szymon Kubala1, Pawel Cwiek1
1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
The New phytologist
|May 22, 2025
概括
该研究将Arabidopsis SWI/SNF染色体重塑复合体 (CRC) 重归类为BAS-A (SWP73A) 和BAS-B (SWP73B) 的子类. SWP73A在辅酶信号传递和发育中具有独特的作用,显示出与SWP73B不同的功能.
科学领域:
- 植物分子生物学 植物分子生物学
- 染色体生物学 染色体生物学
- 遗传学 遗传学 是一个
背景情况:
- 切换缺陷/非发酵糖 (SWI/SNF) 的ATP依赖的染色体重塑复合体 (CRC) 对基因表达,发育和激素信号传递至关重要.
- 阿拉比多普西斯中的SWI/SNF家族包含像SWP73A和SWP73B这样的子单元,其独特的功能尚未完全理解.
研究的目的:
- 为了区分Arabidopsis SWI/SNF CRC中的SWP73A和SWP73B子单元的功能作用.
- 根据子单元的组成和功能提出SWI/SNFCRC的精细分类.
- 调查SWP73子单元对激素信号传递,新陈代谢和植物发育的影响.
主要方法:
- 转录造型和染色体免疫沉 (ChIP) 用于分析基因表达和结合模式.
- 质谱学,酵母双杂交和双分子光补充用于蛋白质-蛋白质相互作用.
- 激素和代谢物分析,以及表型评估,以评估功能后果.
主要成果:
- 识别了包含SWP73A的新型SWI/SNF CRC子类,从而提出了BAS-A和BAS-B分类.
- 含有SWP73A和SWP73B的CRC表现出明显的染色体结合,激素生物合成和代谢效应.
- SWP73A在辅酶信号传递,根部发育,新陈代谢和发芽过程中表现出特定的,不可补偿的作用.
结论:
- SWP73A和SWP73B子单元定义了Arabidopsis中SWI/SNFCRC的不同子类,具有独特的功能性质.
- SWP73子单元的基因重复有助于调节植物过程的共享和专业功能.
- 在特定的发育和信号通路中,SWP73A起着至关重要的作用,SWP73B无法完全取代.
更多相关视频
相关概念视频
Protein Complexes with Interchangeable Parts
2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
Nucleosome Remodeling
9.6K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.6K
Cell Signaling in Plants
5.8K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.8K
Chromatin Modification in iPS Cells
1.9K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.9K
Condensins
3.7K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.7K
The Antenna Complex
6.3K
Plants and other photosynthetic organisms comprise pigments capable of absorption of direct sunlight. These pigments are present in the reaction center - the main site of photochemical reactions as well as in the antenna complex. Under average light conditions, the rate at which reaction center pigments absorb light is far below the electron transport chain's capacity. As a result, the reaction center alone cannot provide enough energy to drive photosynthesis. The photosynthetic efficiency can...
6.3K


