一个单克隆抗体和两聚合物的竞争性吸附到空气-水接口
Elise J Hingst1, Michaela Blech2, Dariush Hinderberger1
1Institute of Chemistry, Physical Chemistry-Complex Self-Organizing Systems, Martin Luther University Halle-Wittenberg, Von-Danckelmann-Platz 4, 06120, Halle (Saale), Germany.
European biophysics journal : EBJ
|May 22, 2025
概括
波洛克萨默188 (P188) 抑制在接口的单克隆抗体 (mAb) 吸附,而聚酸盐20 (PS20) 可以消化新鲜的mAb层. 陈旧的mAb层由于时间依赖的重组而抵抗脱吸.
科学领域:
- 生物物理化学 生物物理化学
- 材料科学 材料科学 材料科学
- 制药科学 制药科学
背景情况:
- 单克隆抗体 (mAbs) 是重要的生物制药,但它们在配方中的稳定性受到界面吸附的挑战.
- 了解mAb在接口上的自我组织是开发稳定的蛋白质药物产品的关键.
研究的目的:
- 为了研究mAbs和常见稳定剂 (poloxamer188 [P188]和polysorbate20 [PS20]) 在空气-水界面上的竞争性吸附.
- 为了确定这些表面活性剂是否可以阻止mAb吸附或消化预吸附的mAb分子.
主要方法:
- 朗穆尔薄膜平衡测量以评估表面压力.
- 滴形张力计用于测量表面张力.
- 红外反射吸收光谱 (IRRAS) 用于分析表面组成和吸附的分子量.
主要成果:
- 在刷牙阶段 (≥0.3 mg/L) 的P188有效抑制mAb吸附.
- 在mAb吸附后添加P188不会导致mAb脱吸.
- PS20可以去吸收新形成的mAb层 (长达2小时),但不能去吸收老化的层 (17小时).
结论:
- P188的相位状态显著影响mAb吸附.
- 在空气-水界面上,mAb的时间依赖的重组增加了对脱吸的抵抗力.
- 在mAb介面膜内的分子间相互作用可能有助于这种抗性增加.
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