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Updated: Sep 20, 2025

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通过SRSF1介导的替代拼接,通过HIF1A/BNIP3/mitophagy轴调节膀癌的进展和对西斯普拉丁的敏感性

Qikai Wu^1,2, Hao Yu^1,2, Huanyou Sun^1,2

¹Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.

Journal of translational medicine

|May 22, 2025

概括

在膀癌 (BCa) 中高SRSF1表达通过激活HIF1A/BNIP3/mitophagy通路来促进瘤进展和西斯普拉丁耐药性. SRSF1是BCa诊断和化疗的潜在生物标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

替代拼接 (AS) 与瘤进展有关.
一个原型瘤基因SRSF1在剪接中起着关键作用.
在膀癌 (BCa) 进展和化学敏感性中SRSF1的作用尚不清楚.

研究的目的:

研究SRSF1在BCa进展中的功能.
确定SRSF1对BCa化学敏感性的影响.
阐明SRSF1在BCa中的作用背后的机制.

主要方法:

定量实时PCR (RT-qPCR) 和西部抹杀用于SRSF1表达.
在体外和体内测试扩散,迁移和瘤发生.
RNA测序,JC-1染色和电子显微镜用于线粒的途径分析.
机械验证的RNA免疫沉降和CUT&RUN试验.

主要成果:

高SRSF1表达与不良的BCa预后相关.
SRSF1促进BCa细胞的进展和对西斯普拉丁的抗性.
通过替代拼接,SRSF1增强了HIF1A的产生,激活了HIF1A/BNIP3/mitophagy轴.
这一轴驱动BCa的进展,并降低了西斯普拉丁的敏感性.

结论:

通过HIF1A/BNIP3/mitophagy通路,SRSF1促进BCa的进展和对西斯普拉丁的耐药性.
SRSF1是BCa诊断和化疗反应的潜在生物标志物.
准SRSF1可能为BCa提供新的治疗策略.

关键词:

另一个替代拼接.膀癌是一种癌症.西斯普拉丁的耐药性线粒细胞衰变 (mitophagy) 是一种神经衰变.在SRSF1中,我们可以使用SRSF1.

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