对一种用于间接合成尿液识别的新型自动化试验的诊断灵敏度和特异性的评估
Laura Franke1, Christian Fuczik2, Dirk K Wissenbach1
1Jena University Hospital, Institute for Forensic Medicine, Friedrich Schiller University Jena, Jena, Germany.
Drug testing and analysis
|May 23, 2025
概括
标准测试难以检测合成尿液. 这项研究评估了特定的标记物,发现结合多种测试,包括尿酸和LC-MS/MS分析,对于准确识别合成样品至关重要.
科学领域:
- 法医毒理学 法医毒理学
- 临床化学 临床化学
- 分析化学 分析化学
背景情况:
- 合成尿很难通过标准样本有效性测试来检测.
- 精确识别合成尿在各种法医和临床应用中至关重要.
研究的目的:
- 评估VDx真尿LD (长时间) 和SD (短时间) 测试在检测合成尿液方面的有效性.
- 评估尿酸,温度和LC-MS/MS分析对合成尿的识别的有用性.
- 为了确定LD和SD标记物的诊断灵敏度和特异性.
主要方法:
- 分析了两种美国合成尿液和1188个现实生活标本,用于LD和SD标记物,尿酸和温度.
- 对47个可疑标本进行10个内源生物分子的LC-MS/MS分析.
- 对直接合成尿液标记物的LC-(HR) MS分析.
主要成果:
- 无论是LD和SD标记器都成功检测到了合成尿液.
- 尿酸和LC-MS/MS分析有效地识别了合成尿.
- LD标志物显示100%的灵敏度和88.9%的特异性,而SD标志物在第1组中具有84.2%的灵敏度和44.4%的特异性.
结论:
- 单一的测试不足以确认真实或合成的尿液.
- 建议同时分析间接的 (LD/SD标记物,尿酸) 和直接的合成尿液标记物.
- 卡本达齐姆在美国的一个合成尿样中被发现.
更多相关视频
04:57Comparative Analysis of Automatic Fecal Analyzer versus Direct Wet Smear Microscopy for Detecting Parasitic Infections in Stool Samples
Published on: April 25, 2025
1.0K
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
10.2K
相关概念视频
01:29Urine Studies I: Urinalysis
1.2K
Urinalysis is a widely used diagnostic test that analyzes urine's physical, chemical, and microscopic characteristics. Healthcare providers use it to detect and monitor various health conditions, including renal disease, urinary tract infections (UTIs), diabetes, and metabolic or systemic disorders.Components of UrinalysisUrinalysis consists of three primary components: physical, chemical, and microscopic examination. Each provides unique insights into the urine sample and, by extension, the...
1.2K
01:26Urine Studies II: Urine Culture and Sensitivity Test
896
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
896
01:33Enzyme-Linked Immunosorbent Assay
17.3K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
17.3K