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相关概念视频

Recombinant DNA01:09

Recombinant DNA

Overview
Recombinant DNA01:09

Recombinant DNA

Overview
The Central Dogma01:20

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...

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相关实验视频

Updated: Jul 17, 2026

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
11:42

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

使用重组DNA技术表达的活性马碳酸化人体因子IX.

H de la Salle, W Altenburger, R Elkaim

    Nature
    |July 18, 1985
    PubMed
    概括

    再组合DNA技术使肝瘤细胞能够产生活跃的人类因子IX. 这一突破为治疗B型血友病,一种严重的出血障碍提供了有希望的途径.

    科学领域:

    • 生物技术是生物技术.
    • 分子生物学分子生物学
    • 血液学 血液学 血液学

    背景情况:

    • 第九因子 (圣诞因子) 对于血液凝固至关重要.
    • 缺少第九因子会导致B型血友病,这是一种遗传性出血障碍.
    • 目前的处理涉及到因子IX缩物,需要改进生产方法.

    研究的目的:

    • 探索重组DNA技术的使用,以产生功能的人类第九因子.
    • 评估基于疫苗病毒的载体对表达复杂蛋白质的疗效.
    • 为了评估不同细胞类型的因子IX的产生.

    主要方法:

    • 一个人类的第九因子cDNA克隆被插入到疫苗病毒载体中.
    • 重组载体被用来感染人类肝瘤细胞和小鼠纤维细胞.
    • 在产生的蛋白质中测量了因子IX活性.

    主要成果:

    • 人类肝瘤细胞成功地产生了完全活跃的第九因子.
    • 无论维生素K水平如何,小鼠纤维细胞都会产生一种不那么活跃的因子IX形式.
    • 这证明了复杂的,经过翻译修改的蛋白质通过重组DNA技术的成功表达.

    更多相关视频

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    Published on: February 2, 2018

    Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice
    08:13

    Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice

    Published on: September 30, 2021

    相关实验视频

    Last Updated: Jul 17, 2026

    High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
    11:42

    High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

    Published on: December 28, 2015

    Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
    09:35

    Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

    Published on: February 2, 2018

    Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice
    08:13

    Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice

    Published on: September 30, 2021

    结论:

    • 基于疫苗病毒的载体可以在真核细胞中有效地表达活性,复杂的治疗性蛋白质,如因子IX.
    • 这种方法对大规模生产血友病B治疗的因子IX具有重大潜力.
    • 该研究强调了用于临床应用的重组蛋白质生产的进步.