长期戒除吗啡会改变精子smRNA的表达,并防止代际表型的传播
Dana Zeid1, Andre B Toussaint2, Carmen Dressler1
1Department of Psychology & Neuroscience, College of Liberal Arts, Temple University, Philadelphia, PA 19122, United States.
Environmental epigenetics
|May 23, 2025
概括
父亲暴露于吗啡可以改变后代的成行为,但在怀孕前90天的禁欲期可以防止这些变化. 特定的精子小RNAs (smRNAs) 与吗啡暴露相关,在父亲戒断后正常化.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 神经科学是一个神经科学.
- 生殖生物学 生殖生物学
背景情况:
- 父亲接触滥用药物可以诱导生殖细胞的表观遗传变化,影响后代的行为.
- 之前的研究表明,暴露于吗啡的父亲产生了与上相关的行为改变的后代.
研究的目的:
- 为了确定吗啡自我管理后的父亲禁欲期是否可以防止与成相关的表型传播给后代.
- 为了确定精子中的特定小RNA (smRNA) 变化,与父亲的吗啡暴露和禁欲有关.
主要方法:
- 雄性大鼠接受了吗啡自给药,随后进行了90天的戒断期.
- 对后代的行为进行了评估,以确定与成相关的表型.
- 来自暴露于吗啡的精子中的小RNA含量,暴露于盐水的精子,暴露于吗啡的戒断精子和暴露于盐水的戒断精子的精子使用测序进行了比较.
主要成果:
- 戒酒90天的后代没有表现出以前观察到的与吗啡有关的行为变化.
- 两种smRNAs,rno-miR-150-5p和一个Snora42/Noc3l注释的snoRNA,与盐水对照相比,在暴露于吗啡的的精子中表达不同.
- 在被暴露于吗啡和被暴露于盐水的男性精子之间没有发现smRNA表达的差异.
结论:
- 90天的父性禁欲期足以防止后代继承与吗啡成相关的内分类型.
- 精子中的特定smRNA与父亲的吗啡暴露相关,这表明跨代表观遗传的机制.
- 怀孕前父亲的禁欲可以减轻后代继承与成有关的行为改变易感性的风险.
相关概念视频
01:41Spermatogenesis
102.3K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.3K
01:20Opioid Analgesics: Morphine and Other Natural Cogeners
192
Opioids are a class of drugs that mimic endogenous opioid peptides and act on opioid receptors, and help in pain relief. These compounds are classified as natural, synthetic, or semi-synthetic. Natural opioids, like morphine, codeine, and thebaine, are derived from the opium poppy plant (Papaver somniferum or Papaver album) and are termed opiates. Synthetic opioids are artificial, while semi-synthetic opioids combine natural and synthetic compounds. Morphine, a prototypical opioid, possesses a...
192
02:30Genomic Imprinting and Inheritance
34.2K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
34.2K
01:15Opioid Analgesics: Synthetic and Semisynthetic Opioids
251
Synthetic and semisynthetic opioids are pivotal in pain management and tackling opioid addiction. Semisynthetic opioids, including morphinans (morphine derivatives), oxycodone, oxymorphone, hydrocodone, and hydromorphone, have improved pharmacokinetic profiles compared to morphine. Additionally, heroin and 6-MAM (6-Monoacetylmorphine) show better CNS penetration than morphine due to heightened lipid solubility. Hydromorphone, a potent opioid, undergoes hepatic metabolism to form the active...
251