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主体和共生细菌在单细胞分辨率上协同对抗机会性病原体

Sheng Zhang¹, Ziguang Wang², Anqi Liu¹

¹School of Plant Protection, Anhui Agricultural University, Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Province Key Laboratory of Resource Insect Biology and Innovative Utilization, Hefei, 230036, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|May 23, 2025

概括

果虫和乳植物植物菌 (Lactiplantibacillus plantarum) 结成一个联盟,对抗致病性Serratia marcescens. 这种宿主-微生物相互作用塑造了细菌群落和耐药性,为共生提供了洞察力.

科学领域:

微生物学微生物学
宿主-微生物相互作用
系统生物学系统生物学

背景情况:

微生物群落是复杂的,监管机制不太清楚.
主体-微生物和微生物-微生物的相互作用对生态系统动态至关重要.

研究的目的:

研究涉及Drosophila,Lactiplantibacillus plantarum和Serratia marcescens的三种物种相互作用的分子机制.
阐明宿主-微生物联盟如何影响竞争和殖民抵抗.

主要方法:

使用了一个整合的三种物种模型系统 (Drosophila,L. plantarum,S. marcescens).
在幼虫和细菌群体中分析了转录重编程.
在单细胞分辨率下检查了种群动态和毒性演变.

主要成果:

菌促进了L. plantarum的生长,从而赋予了对S. marcescens的殖民抵抗力.
S. marcescens采用适应性策略,包括感知乳酸盐和演变的毒性异质.
L. plantarum表现出更广泛的乳酸生成异质性,以支持宿主联盟.

结论:

主体-共生-病原体共生涉及复杂的调节网络和适应性进化.
单细胞分辨率揭示了细菌社区操纵和耐药异质性的机制.
这些发现推进了微生物生态学和宿主微生物相互作用的基本概念.

关键词:

细菌单细胞RNA-seqq.异质性的异质性代谢适应代谢适应病原性是一种病原性.

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