斯特罗姆类细胞和视网膜色素上皮层通过 Galectin-1-依赖和独立的途径调节胆芽
Magali E Ridano1,2, Christin Hanke-Gogokhia3, Guillermo L Lehmann3
1Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
概括
加勒-1 (Gal1) 对于保持视网膜色素表皮质 (RPE) 和胆管状细胞的健康至关重要. 这项研究揭示了Gal1在调节胆道血管生长中的作用,影响疾病中的RPE/胆道功能.
科学领域:
- 眼科医生 眼科 眼科
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 外视网膜功能依赖于视网膜色素表皮质 (RPE) 和胸膜.
- 维持RPE/choroid的细胞间网络尚未完全被理解.
- 加勒-1 (Gal1) 涉及血管生成和血管疾病,如与年龄相关的黄斑变性.
研究的目的:
- 研究外视网膜中的Gal1表达.
- 在生理和病理条件下确定Gal1在RPE/choroid中的调节作用.
主要方法:
- 在野生类型和Gal1-缺乏 (Lgals1-/-) 小鼠中的Gal1表达的分析.
- 评估RPE/状腺超结构和基因表达.
- 冠状腺扩散体生长试验.
- 在体外研究,在低氧条件下使用类似树叶细胞和重组Gal1.
主要成果:
- 1主要存在于RPE/状腺 stromal 细胞中.
- 缺少Gal1会改变RPE/胆体结构和基因表达,减少胆体的发芽.
- 再组合的Gal1在缺氧条件下促进胆道芽.
- 状细胞在体外调节血管新生基因表达.
- 在正常氧化时,RPE会以角分泌Gal1,在低氧化时则以基侧分泌Gal1.
结论:
- 流体样细胞和RPE是胆膜中关键的Gal1来源.
- 在健康和病态状态下,Gal1在维持RPE/胆管平衡中起着独特的作用.
- Gal1影响胆道血管生成和RPE功能.
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