肠病原性大肠杆菌诱导Entamoeba histolytica通过降低引力来促进纤维素的超扩散运动
Yuanning Guo1, Jun Ye2, Ariel Shemesh3
1Department of Genetics and Developmental Biology, Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel.
PLoS pathogens
|May 23, 2025
概括
肠病原性大肠杆菌的存在增加了Entamoeba histolytica的运动性,增加了其引起组织损伤的潜力. 这种相互作用改变了寄生虫的细胞行为和机械力量,影响了阿米比亚斯病原性.
科学领域:
- 寄生虫学的寄生虫学
- 微生物学 微生物学
- 细胞生物物理学 细胞生物物理学
背景情况:
- 由Entamoeba histolytica引起的阿米比亚斯是一种重要的全球健康问题.
- 肠道细菌对E. histolytica侵入性潜力的影响尚未完全理解.
研究的目的:
- 调查肠病原性大肠杆菌如何影响E. histolytica的运动性,机械力和侵入性.
- 阐明物理相互作用在寄生虫病原性中的作用.
主要方法:
- 使用微柱阵列系统进行精确的测量.
- 使用实时成像来观察E. histolytica的行为.
- 分析了F-actin局部化和拉力作用在纤维内素涂层基板上的作用.
主要成果:
- 与大肠杆菌的同时化显著增强了E. histolytica的运动性 (超扩散运动).
- 增加的运动性导致了更广泛的分散和更大的组织损伤潜力.
- 观察到减少了F-actin依赖的引力,但在上细胞质中增加了F-actin.
结论:
- 细菌的存在调节了E. histolytica的物理相互作用和细胞行为.
- 这些变化增强了寄生虫的运动性和毒性.
- 提供了对阿米比亚斯病原性的机制性见解.
相关概念视频
01:27Chemotaxis in E. coli
1.0K
Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
1.0K
01:24Adherens Junctions
6.9K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
6.9K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.7K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.7K
01:10Intracellular Movement of Viruses and Bacteria
3.7K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.7K
01:24Protein Diffusion in the Membrane
6.0K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
6.0K