相关实验视频

Updated: Sep 20, 2025

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Published on: March 11, 2017

通过调节涉及miR-548m/IGF1轴的巨细胞极化,CiR-EIS减轻了与代谢功能障碍相关的脂肪肝炎

Xiaoman Chen¹, Zhiping Wan², Lili Wu²

¹Department of Infectious Diseases, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China; Guangdong Provincial Key Laboratory of Liver Disease Research, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China; Guangdong Provincial Key Laboratory of Diabetology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.

Clinical nutrition (Edinburgh, Scotland)

|May 23, 2025

概括

循环RNAciR-EIS通过影响miR-548/IGF1轴来调节代谢功能障碍相关的脂肪肝炎 (MASH) 中的大细胞两极分化,从而具有作为诊断和治疗标的潜力.

科学领域:

肝病学肝病学是一种肝病学.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

代谢功能障碍相关的脂肪肝炎 (MASH) 是一个日益严重的公共卫生问题.
巨细胞两极分化在MASH进展中起着至关重要的作用.
循环RNA ciR-EIS在MASH相关的巨细胞极化中的功能尚不清楚.

研究的目的:

为了研究ciR-EIS对MASH中的巨细胞极化的影响.
探索涉及miR-548/IGF1轴的潜在分子机制.

主要方法:

免疫光和现场杂交用于ciR-EIS局部化.
使用THP-1-衍生巨细胞 (TDMs) 进行体外研究,以评估ciR-EIS功能.
流细胞计和RT-qPCR用于分析巨细胞极化标记物.
在HepG2细胞中检测脂质积累的Bodipy测定.
对与ciR-EIS,IGF1和MASH严重程度相关的临床数据的回顾性分析.

主要成果:

在MASH患者和M1巨细胞中,CiR-EIS的调节下降,但在M2巨细胞中调节上升.
在TDM中ciR-EIS的过度表达减少了M1标志物 (CD86,IL-1β,TNF-α) 和增加了M2标志物 (CD163,CD206).
CiR-EIS降低了肝细胞脂质积累和肝星细胞增殖.
通过miR-548m/IGF1轴,CiR-EIS调节了巨细胞的极化.
血清IGF1水平与ciR-EIS正相关,与MASH严重性得分 (APRI,FIB-4) 逆相关.

结论:

通过miR-548/IGF1通路调节巨细胞极化,减轻MASH病理.
作为一个潜在的诊断生物标志物和MASH的治疗点,CiR-EIS显示出了希望.

关键词:

据了解,Hsa_circ_000888282 的数据来源于胰岛素类生长因子1 1 巨细胞的两极分化与代谢功能障碍相关的脂肪肝炎.与代谢功能障碍相关的脂肪性肝病.一个米R-548m.

更多相关视频

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

Published on: April 26, 2019

相关概念视频

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...