内皮细胞药物递送的依赖性对单分散微气泡动态的依赖性
Yuchen Wang1, Hongchen Li1, Bram Meijlink1
1Biomedical Engineering, Department of Cardiology, Cardiovascular Institute, Erasmus MC, Rotterdam, 3015 GD, the Netherlands.
概括
选择正确的微气泡大小是有效的超声中介药物输送的关键. 2.2微米的微泡显示了细胞吸收和声波穿孔的最佳结果.
科学领域:
- 生物医学工程 生物医学工程
- 声学医学是一种声学医学.
- 药物输送系统 药物输送系统
背景情况:
- 微泡的超声无声化增强了局部药物输送的血管透性.
- 统一的微气泡声学反应和理解生物物理机制对于有效的治疗结果至关重要.
研究的目的:
- 研究单分散微气泡大小和动态对细胞反应和药物输送的影响.
- 确定最佳的微气泡大小,以增强超声波和细胞内药物吸收.
主要方法:
- 使用微流体制造的单分散微气泡 (1.5-2.9微米半径).
- 在无声化 (2 MHz) 时使用超高速成像 (每秒1000万) 捕获实时微气泡振荡.
- 通过共聚焦显微镜 (2D和3D) 观察到内皮细胞中的细胞反应和药物吸收.
主要成果:
- 2.2微米的微泡,在2MHz的共振,显示了最高的声波穿孔率 (75%),最大的膜穿孔 (78微米2),和最大的细胞内药物吸收.
- 1.5微米的微气泡具有类似的声波穿孔 (73%),但穿孔较小 (20微米2) 和更高的超内皮输送 (64%).
- 微气泡产生的剪压被确定为声波穿孔的主要驱动因素.
结论:
- 微气泡大小的选择对于优化微气泡中介药物输送至关重要.
- 2.2μm的微泡是通过声波穿孔最大限度地提高细胞内药物输送的最佳选择.
- 这些发现支持单分散微气泡用于向治疗的安全临床转化.
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