在Populus alba×P.glandulosa中,PagWRKY11调节了叶子形态和盐敏感性
Shixian Liao1, Yuting Wang1, Nan Jiang1
1State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.
Phytochemistry
|May 23, 2025
概括
在树中过度表达PagWRKY11基因改变了叶子形状,增加了盐敏感性. 这种 WRKY 转录因子对植物对盐应激的防御机制产生负面影响.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 遗传学 是一个遗传学.
背景情况:
- WRKY转录因子 (TFs) 对植物发育和应激反应至关重要.
- 大多数WRKYTFs在84K (Populus alba × P. glandulosa) 中关于叶子形态和盐应激的特定作用仍然在很大程度上未知.
研究的目的:
- 调查PagWRKY11基因在调节树叶形态和盐应激反应中的功能.
- 了解PagWRKY11对植物发育和应力耐受性影响的分子机制.
主要方法:
- 转基因树系产生过度表达的 PagWRKY11.
- 转基因和野生类型 (WT) 植物的叶子和芽形态的表型分析.
- 在盐应激下对抗氧化酶活性 (SOD,POD),H2O2和MDA积累的生物化学测定.
- 酵母的一种混合测试以确定DNA结合的特异性.
主要成果:
- 与WT相比,转基因树表现出更窄,更光滑的叶子和明显的顶端芽形态.
- 过度表达PagWRKY11导致SOD和POD活性和基因表达减少,同时在盐应激下增加H2O2和MDA积累,表明盐敏感性增加.
- PagWRKY11过度表达增加了叶子的水分流失率,并通过影响水分保留,对盐应激反应产生了负面影响.
- PagWRKY11 特别与 W-box 元素结合.
结论:
- 过度表达PagWRKY11通过降低抗氧化剂防御基因和影响水分保留来增强树的盐敏感性.
- PagWRKY11在调节叶子形态发生过程中发挥作用,并对盐应激反应产生负面影响.
- 这些发现提供了关于PagWRKY11分子机制的见解,以及开发耐盐品种的潜力.
更多相关视频
05:22Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
Published on: January 20, 2023
1.8K
09:54The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
Published on: October 5, 2016
8.9K
相关概念视频
Responses to Salt Stress
13.4K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.4K
Regulation of Transpiration by Stomata
29.2K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
29.2K
Adaptations that Reduce Water Loss
26.4K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
26.4K
Morphogenesis
28.9K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
28.9K
Primary and Secondary Growth in Roots and Shoots
58.2K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
58.2K
Cell Signaling in Plants
5.8K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.8K
