解读人类男性生殖系从胚胎到成年发展的调节网络
Jun Chen1, Xinyan Yang2, Manman Cui3
1Department of Biochemistry and Molecular Biology, Shenzhen University Medical School, Shenzhen, Guangdong 518060, PR China.
概括
这项研究绘制了人类男性生殖细胞从胚胎到老年发展的地图,确定了关键的信号通路和转录因子,如JUN和MYC,它们调节干细胞的维护和分化. 它还揭示了在非阻塞性阿佐精子症中失调的基因,用于潜在的诊断.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 基因组学就是基因组学.
背景情况:
- 人类男性生殖系的发育对于生育至关重要,但尚未完全表征.
- 了解精子干细胞动态对于终身精子生产至关重要.
研究的目的:
- 构建一个关于人类男性生殖细胞发育的全面地图.
- 确定监管网络和生殖线发展的关键因素.
- 发现与异常非阻塞性精子缺血症 (iNOA) 相关的基因.
主要方法:
- 整合了来自92,488个人类丸细胞的单细胞RNA测序 (scRNA-seq) 数据.
- 分析从胚胎阶段到老年人的发育轨迹.
- 询问来自iNOA患者的scRNA-seq数据集.
主要成果:
- 建立了一个详细的人类男性生殖细胞图谱.
- 在早期生殖细胞发育过程中确定了一个WNT6-FZD3/LRP6-JUN/MYC信号轴.
- JUN和MYC被认为是抑制精子分化的抑制剂.
- 发现ANGPTL信号维持了精子干细胞.
- CAPN3,FTMT,IZUMO2和LACE1在iNOA患者的圆精子中被确定是显著下调的.
结论:
- 该研究为人类男性生殖细胞发育提供了全面的资源.
- 鉴定的调控因素为控制生殖线发育和干细胞维护提供了洞察力.
- 发现的iNOA调节失调的基因可以作为临床诊断和理解男性不孕症的生物标志物.
相关概念视频
01:15Development of the Sexual Organs in the Embryo and Fetus
1.5K
Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
1.5K
02:19The Y Chromosome Determines Maleness
6.9K
The Y chromosome is a sex chromosome found in several vertebrates and mammals, including humans. In addition to 22 pairs of autosomes, the human males have one X chromosome and one Y chromosome. In these organisms, the presence or absence of the Y chromosome determines the development of male traits.
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size....
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size....
6.9K
01:26Testosterone: Functions and Regulation
1.1K
The intricate hormonal interplay essential for male reproductive health begins with the release of gonadotropin-releasing hormone (GnRH) by the hypothalamus. This hormone prompts the pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). LH targets the Leydig cells in the testes, stimulating them to produce and release testosterone. In concert with testosterone, FSH acts on the Sertoli cells within the seminiferous tubules to facilitate the release of...
1.1K
02:23Master Transcription Regulators
7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K
01:41Spermatogenesis
103.5K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
103.5K
02:24Regulation of Expression Occurs at Multiple Steps
3.2K
3.2K