染色体尺度组合和功能注释的复杂虫寄生虫Eimeria acervulinaina的功能注释
Subodh K Srivastava1, Carolyn C Parker2, Peter C Thompson2
1USDA ARS, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, East, Building 1040, 10300 Baltimore Ave., Beltsville, MD, 20705, USA. Subodh.k.Srivastava@usda.gov.
Scientific data
|May 23, 2025
概括
研究人员已经创建了一个高质量的基因组组装Eimeria acervulina,寄生虫引起菌病. 这种基因组资源将有助于开发新的抗菌药物和疫苗.
科学领域:
- 基因组学就是基因组学.
- 寄生虫学的寄生虫学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 猿复合体寄生虫导致全球疾病负担显著.
- 埃梅里亚 (Eimeria acervulina) 是一个关键的家禽寄生虫,负责菌病.
- 完整的基因组组合对于识别耐药性标记物和疫苗点至关重要.
研究的目的:
- 为了生成高质量的染色体规模的基因组组合Eimeria acervulina.
- 进行全面的基因预测和功能注释.
- 为了进行特定阶段的转录组分析.
主要方法:
- 使用牛津纳米孔和Illumina技术进行测序.
- Hi-C近距离链接库的构建.
- 染色体尺度组装使用MaSuRCA组装器.
- 使用BRAKER3,GeneMark-ETP和AUGUSTUS管道进行基因预测.
- 转录组分析.
主要成果:
- 一个52 Mb的基因组组合,有15个染色体和99%的BUSCO完整性.
- 预测了7,621个基因,其中60.97%具有Pfam功能,25.74%被赋予GO术语.
- 识别了9761个特定阶段的转录.
结论:
- 生成的基因组组装和转录组数据为Eimeria acervulina提供了一个基础资源.
- 这一资源将有助于发现抗菌药物和疫苗开发的新目标.
- 允许未来对寄生虫生物学和控制策略的研究.
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