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精子胺降低了ISGylation,并增强了ISG15-USP18的相互作用

Haruka Etori¹, Riko Asoshina¹, Takayuki Obita²

¹Department of Food and Health Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, 813-8582, Japan.

Scientific reports

|May 23, 2025

概括

发现精氨酸和精氨酸等多氨酸可以减少干扰素刺激的15 (ISG15) 基因结合 (ISGylation). 精子胺还增强了ISG15和USP18之间的相互作用,可能调节免疫反应.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

干扰素刺激的15 (ISG15) 基因结合 (ISGylation) 对于先天免疫非常重要,由干扰素和感染激活.
超过1100种蛋白质被ISG化,ISG15被像USP18这样的蛋白质酶去除,高ISG化可能会影响COVID-19.
营养素,如多氨酸和ISGylation之间的关系在很大程度上是未知的.

研究的目的:

研究多氨酸,特别是精氨酸和精氨酸对ISGylation的影响.
探索这些营养素影响ISGylation通路的潜在机制.

主要方法:

用干扰素-α,精氨酸或精氨酸治疗了MCF10A和A549细胞系.
测量了蛋白质表达水平和ISGylation状态.
分析了精子胺,ISG15和USP18之间的直接相互作用.

主要成果:

精氨酸和精氨酸都显示出对ISGylation的剂量依赖性减少.
观察到精氨酸与ISG15和USP18直接相互作用.
精子胺增强了ISG15和USP18之间的相互作用,表明减少ISGylation的机制.

结论:

多氨酸,精氨酸和精氨酸,可以显著降低ISGylation.
精氨酸与ISG15和USP18的相互作用表明它在调节依赖ISGylation的免疫反应方面发挥了作用.
这些发现为探索ISGylation相关过程中的营养干预开辟了道路.

关键词:

在ISGylation中进行化.干扰素干扰素干扰素干扰素刺激基因 15 kDa 聚胺是一种聚胺.精子胺是什么精子胺是什么

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