相关实验视频
Updated: Sep 20, 2025
06:48
CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
7.0K
在DNA损伤依赖的转录调节中,IncRNA-ZFAS1是一个新兴的门卫
Jiena Liu1, Qing Lu1, Zixuan Fan1
1Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 511436, P. R. China.
概括
长非编码RNAZFAS1对于DNA损伤修复和细胞存活至关重要. 它的缺失会损害核酸切除修复,并在小鼠中引起脏发育不良.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在细胞过程中的角色.
- 由外部刺激诱导的lncRNAs的特定功能,特别是在DNA损伤反应 (DDR) 中,仍然在很大程度上未被探索.
研究的目的:
- 为了研究 lncRNA 转录和 DNA 损伤反应之间的联系.
- 确定涉及DDR途径的特定lncRNA及其作用机制.
主要方法:
- 鉴定和表征 lncRNA ZFAS1 作为对DNA损伤的反应.
- 分析ZFAS1在细胞循环停止,转录调节和DNA修复中的作用.
- 研究ZFAS1与RNA聚合酶II (RNAPII) 酸化状态的相互作用.
- 使用淘汰赛小鼠模型评估ZFAS1功能,评估核酸切除修复 (NER) 和发育.
主要成果:
- 鉴定出 lncRNA ZFAS1 对于细胞循环停止,转录调节和在基因毒性压力后的 DNA 修复至关重要.
- ZFAS1促进RNAPII酸化的动态变化,特别是在转录启动部位周围,促进DNA修复.
- 显著的转录关闭和增加的RNAPII-Ser2P参与对于修复和生存至关重要.
- ZFAS1淘汰赛小鼠表现出受损的NER,并发展出功能障碍,表明其在发育中的作用.
结论:
- 通过调节转录和促进DNA修复机制,ZFAS1在DNA损伤反应中发挥着至关重要的作用.
- 该研究强调了lncRNAs在维护基因组完整性和预防与DDR缺陷相关的发育障碍方面的重要性.
相关概念视频
02:04Co-activators and Co-repressors
7.6K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.6K
02:23Master Transcription Regulators
7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K
02:37DNA Damage can Stall the Cell Cycle
9.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.3K
02:36RNA Polymerase II Accessory Proteins
9.6K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.6K
02:37Restarting Stalled Replication Forks
5.9K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.9K
02:26NF-κB-dependent Signaling Pathway
7.9K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.9K