锡斯-非蛋白:真实发生及其功能作用
Jane S Richardson1, Lizbeth L Videau1, Christopher J Williams1
1Department of Biochemistry, Duke University, Durham, North Carolina, USA.
概括
Cis-nonPro是罕见的,但在蛋白质结构中具有功能关键性,通常在碳水化合物加工酶中发现. 严格的分析证实了它们的真实存在和生物重要性.
科学领域:
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 在proline之前的cis很常见 (5%),但在蛋白质结构中,在其他残留物 (cis-nonPro) 之前的cis很少见.
- 真正的cis-nonPro发生对于生物功能至关重要,包括酶活性位和二硫化键.
- 由于晶体学数据 (2006-2015) 中电子密度较低,经常出现不合理的cis-nonPro赋值.
研究的目的:
- 通过使用高分辨率的晶体学数据和严格的质量过来识别真正的cis-nonPro事件.
- 调查已识别的cis-nonPro的功能作用和结构上下文.
- 突出之前不太强调的cis-nonPro的方面,例如它们与有序结构和碳水化合物相关蛋白质的关联.
主要方法:
- 利用了高分辨率的晶体学数据.
- 在残留水平上应用了严格的质量过,以最大限度地减少假阳性.
- 分析了一大批真正的cis-nonPro实例样本,以确定功能和结构模式.
主要成果:
- 开发了一种程序,以高准确度识别真正的cis-nonPro.
- 调查了cis-nonPro.的各种功能角色和结构上下文.
- 观察到 cis-nonPro 主要发生在有序的蛋白质结构中 (除了附近的二硫化物).
- 在参与碳水化合物加工或结合的蛋白质中发现了显著的cis-nonPro度 (CAZy数据库).
结论:
- 真正的cis-nonPro是罕见的,但对于特定的生物功能至关重要.
- 严格的数据过对于准确识别cis-nonPro. 的必要.
- Cis-nonPro与有秩序的结构和与碳水化合物相关的蛋白质密切相关.
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