赫迪奥蒂斯 (Hedyotis chrysotricha) 水性提取物通过肝细胞核因子4α调节抑制B型肝炎表面抗原和病毒复制
Yina Yu1, Haoyang Hu1, Yichun Zhang1
1State Key Laboratory for Diagnosis and Treatment of Infectious Disease, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310000, Zhejiang, PR China.
概括
黑花的水性提取物 (HCM) 有效地抑制乙型肝炎病毒 (HBV) 的复制和转录. 它的机制涉及激活PI3K-AKT和MAPK-ERK1/2通路,抑制HNF4α活性以对抗慢性HBV感染.
科学领域:
- 植物化学 植物化学
- 病毒学 病毒学
- 药理学 药理学是指药理学的学科.
背景情况:
- 慢性乙型肝炎病毒 (HBV) 感染是一个全球性的健康挑战,治疗选择有限.
- 传统中医 (TCM) 为慢性乙型肝炎提供了潜在的治疗策略.
- 赫迪奥蒂斯 (Hedyotis chrysotricha,简称HCM) 的水性提取物显示出抗HBV活性的前景,但其机制尚不清楚.
研究的目的:
- 为了评估HCM的水性提取物对抗HBV的疗效.
- 阐明HCM抗HBV作用背后的药理机制.
主要方法:
- 使用UPLC-Triple-TOF/MS和HPLC-MS/MS进行HCM的化学分析.
- 在体外和体内评估HBV细胞系中的抗HBV活性和转基因HBV小鼠模型.
- 通过网络药理学,分子对接和转录学来探索机制.
主要成果:
- 在HCM中发现了19个主要的化学成分,包括新型化合物如异胺和单胺.
- HCM显著抑制了HBV复制和转录,降低了HBsAg水平和潜在的ccccDNA.
- 机制研究表明PI3K-AKT和MAPK-ERK1/2通路的激活,导致HNF4α抑制.
结论:
- HCM水性提取物通过抑制病毒复制和转录,显示出显著的抗HBV作用.
- 抗HBV机制涉及PI3K-AKT和MAPK-ERK1/2通路,这些通路负面调节HNF4α.
- HCM代表了慢性乙型肝炎治疗的潜在治疗剂.
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