通过分子印制的电化学传感器在提中确定回素:验证和与色谱技术进行比较
Melek Tunc-Ata1, Ezgi Zekiye Akturk2, Muath Njjar3
1Department of Physiology, Health Science, Pamukkale University, 20170 Denizli, Turkey.
Food chemistry
|May 24, 2025
概括
一个新的分子印记聚合物传感器可以在草药产品中准确检测回素 (RTS),一种有毒的pyrrolizidine类化合物 (PA). 这种电化学方法为监测PA污染和确保消费者安全提供了可靠的替代方案.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
- 毒理学 毒理学 毒理学
背景情况:
- 罗利胺类化合物 (PA) 毒性对公共健康构成重大风险,因植物产品消费增加而放大.
- 作为一种强有力的PA,Retrorsine (RTS) 与严重的肝损伤,突变性和DNA交叉链接有关.
- 对草药产品中PA污染的精确监测对于公共健康和安全至关重要.
研究的目的:
- 开发一种实用且灵敏的电化学传感器,用于检测逆向 (RTS).
- 通过循环电压测量和方波电压测量来探索RTS的电化学行为.
- 在真实世界样本中验证传感器的性能,例如提,并将其与已确定的方法进行比较.
主要方法:
- 在玻璃碳电极上制造分子印记聚合物传感器 (MIPs-GCE).
- 使用循环电压计对RTS进行电化学表征.
- 使用方形波电压计对RTS进行定量检测.
- 验证传感器的准确性和精度,使用西红花样本和LC-MS/MS分析.
主要成果:
- 对于RTS,MIPs-GCE传感器的线性检测范围为0.052 nM.
- 实现了0.02869nm的低检测极限 (LOD).
- 传感器准确地检测到红衣草样本中的RTS,结果与LC-MS/MS数据密切匹配.
- 与28种其他PA化合物相比,证明了高选择性.
结论:
- 开发了一种用于RTS检测的新,可靠和简单的电化学传感器.
- 该传感器为PA监测提供了传统色谱方法的实用和敏感的替代方案.
- 这一进步提高了确保草药产品安全的分析能力.
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