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在患有自身免疫性甲状腺疾病的患者中增加了膜结合的TGF-β1,GITR和GITR连接体的表达
Fumiaki Hayashi1, Naoya Inoue1, Yoshinori Iwatani1
1Department of Clinical Laboratory and Biomedical Sciences, The University of Osaka, Graduate School of Medicine, Yamadaoka 1-7 Suita, Osaka 565-0871, Japan.
调节性T (Treg) 细胞是自身免疫性甲状腺疾病 (AITD) 的关键. 它们的功能和与甲状腺组织中的TGF-β1,GITR和GITRL的相互作用对AITD的发展至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 内分泌学 在内分泌学.
- 病理学 病理学 病理学
背景情况:
- 调节性T (Treg) 细胞对于维持自我耐受性至关重要.
- 在自身免疫性甲状腺疾病 (AITD) 中,Treg细胞透到甲状腺组织.
- 涉及Treg细胞的特定分子相互作用在AITD病原体中的作用需要进一步阐明.
研究的目的:
- 研究膜结合转化生长因子-β1 (mTGF-β1) 和葡萄糖皮质激素诱导的瘤亡因子受体相关蛋白 (GITR) 在AITD患者的Treg细胞上的表达.
- 评估甲状腺组织中GITR连接体 (GITRL) 的局部化.
- 了解在AITD中mTGF-β1,GITR,TGF-β1受体II (TGF-βRII) 和GITRL之间的潜在相互作用.
主要方法:
- 来自AITD患者和健康对照者的甲状腺组织和外周血液的免疫组织化学分析.
- 在Treg和效应T (Teff) 细胞群中量化mTGF-β1+和GITR+细胞.
- 在甲状腺细胞中评估GITRL表达.
主要成果:
- 与AITD患者的外周血液相比,甲状腺内的Treg细胞中观察到mTGF-β1+细胞的比例更高.
- 在AITD患者甲状腺中的Treg和Teff细胞中,GITR+细胞的比例在甲状腺中的Treg和Teff细胞中都增加,而不是周围血液.
- 与健康受试者相比,AITD患者的甲状腺细胞中GITRL表达显著更高.
结论:
- mTGF-β1,GITR,TGF-βRII和GITRL的平衡和相互作用,特别是甲状腺细胞GITRL的表达,是AITD病变发生的潜在关键因素.
- 这些分子参与者可能会影响Treg细胞的功能,并促进甲状腺的自身免疫过程.
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