柏柏林化物通过PI3K/AKT信号通路调节巨细胞极化,缓解了肠道炎症和改善了手术后的内脏
Yuxin He1, Shuchang Liu1, Ting Zheng2
1Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
概括
柏柏林化物 (BBR) 通过减少炎症和恢复肠道运动性,有效地治疗术后内脏 (POI). 它通过PI3K/AKT通路抑制M1巨细胞极化而起作用.
科学领域:
- 药理学 药理学是指药理学的学科.
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
背景情况:
- 手术后阴道 (POI) 是腹部手术后的一种常见并发症,其特点是胃肠道运动能力受损.
- 目前对POI的治疗有局限性,需要探索新型治疗剂.
研究的目的:
- 调查柏柏林化物 (BBR) 对POI的治疗作用.
- 阐明BBR减轻POI行动的潜在机制.
主要方法:
- 通过肠道操纵 (IM) 诱导POI的小鼠模型.
- 胃肠道过渡 (GIT),细胞因子表达,白细胞透和巨细胞两极分化在IM后被评估.
- 使用骨髓衍生的巨细胞 (BMDMs) 进行了网络药理学和体外研究,以探索BBR的作用机制,重点关注PI3K/AKT信号通路.
主要成果:
- 在POI小鼠中,BBR显著改善了GIT,减少了炎症标志物,并降低了M1巨细胞极化.
- 网络药理学表明,BBR的抗炎作用通过PI3K/AKT通路进行介导.
- 在体外,BBR通过降低PI3K/AKT信号的调节来抑制M1巨的两极分化,PI3K激动剂可以逆转这种效应.
结论:
- 柏柏林化物 (BBR) 有效地缓解肠道炎症,并恢复POI的肠道过渡.
- 通过调节PI3K/AKT信号通路,BBR通过抑制M1巨细胞极化来发挥其治疗作用.
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