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进化保存的RLF是一种细胞染色体b₅类的血结合蛋白,它调节了Marchantia polymorpha中的器官发育

Kentaro P Iwata¹, Takayuki Shimizu^2,3, Yuuki Sakai¹

¹Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai, Kobe, 657-8501, Japan.

The New phytologist

|May 25, 2025

概括

减少侧根形成 (RLF) 蛋白质与血红素结合,这对其在植物器官发育中的功能至关重要. 在Arabidopsis thaliana和Marchantia polymorpha中这种保存的功能表明植物进化中的古代角色.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
进化生物学进化生物学

背景情况:

减少侧根形成 (RLF) 是Arabidopsis thaliana中的一种独特的细胞染色体b5类血结合域 (Cytb5-HBD) 蛋白质,对侧根发育至关重要.
与其他参与新陈代谢的Cytb5-HBD蛋白质不同,RLF在器官生成中的特定作用仍然不清楚.
对于RLF功能的血结合的必要性以及对物种间其器官发育作用的保护是未知的.

研究的目的:

为了调查血红素结合是否对于Arabidopsis thaliana的RLF功能至关重要.
为了确定其他植物物种的RLF骨科是否调节器官发育.
探索RLF在植物有机发生中的作用的进化保护.

主要方法:

对于RLF及其Marchantia polymorpha ortholog (MpRLF) 的体外血结合试验.
在RLF中保存的histidine残留物的局部定向突变发生.
使用MpRLF补充了Arabidopsis rlf突变物.
在Marchantia polymorpha.中对功能丧失突变物 (mprlfge) 的分析.

主要成果:

RLF在体外结合血红素,保存的胺残留物对血红素结合和生物功能都至关重要.
此外,MPRLF还结合了血红素,并挽救了Arabidopsis rlf突变的横向根形成缺陷.
在MpRLF (mprlfge) 中的功能丧失突变损害了Marchantia polymorpha. gemma杯的状体生长和生殖器官形成.
MpRLF对于M. polymorpha的植物和生殖发育至关重要.

结论:

血结合对于RLF功能在Arabidopsis thaliana的器官发育中至关重要.
RLF对器官发育的调节作用在植物Marchantia polymorpha中得到保留.
通过RLF介导的氧化还原系统在各种植物系中得到保护,并被选择用于植物和种子植物的器官发育.

关键词:

阿拉比多普西斯塔利亚纳.马尔坎蒂亚多态植物 (Marchantia polymorpha) 是一个多态植物.在 RLF 中,RLF 是 RLF.细胞染色体b5类似的血红素结合域杰玛玛 Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma Gemma 杰玛杯是一杯.侧侧的根形成.生殖器官是一种生殖器官.

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