来自形成石头的尿蛋白通过氧化修饰促进氧化酸结晶,生长和聚合
Sudarat Hadpech1, Paleerath Peerapen1, Sakdithep Chaiyarit1
1Medical Proteomics Unit, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Journal of advanced research
|May 25, 2025
概括
氧化修饰的尿蛋白显著增加氧酸盐 (CaOx) 结石形成的风险. 这些改变的蛋白质促进CaOx结晶,生长和聚合,与健康个体的蛋白质不同.
科学领域:
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学.
- 腎臟病學 (nephrology) 是一種醫學.
背景情况:
- 目前的结石风险评估主要使用小分子和pH的尿度.
- 泌尿大分子,特别是氧化蛋白在结石形成中的作用尚不清楚.
研究的目的:
- 调查尿道宏分子,特别是氧化修饰蛋白在氧酸盐 (CaOx) 结石风险中的作用.
- 为了比较健康个体和CaOx结石形成者的尿蛋白.
主要方法:
- 从健康个体和CaOx石块形成者中净化复杂的尿蛋白 (蛋白质组).
- 使用了水晶分析,定量蛋白质组学 (纳米LC-ESI-LTQ-Orbitrap-MS/MS),生物信息分析和ELISA.
- 分析了物理化学特性和蛋白质氧化水平.
主要成果:
- 形成石头的蛋白质组促进了CaOx结晶,生长和聚合,而健康的蛋白质组抑制了这些过程.
- 在石头形成者的尿液中,氧化修饰蛋白质,特别是氨酸和氨酸氧化增加了约2.5倍.
- 定量蛋白质组学确定了不同的蛋白质配置文件,氧化蛋白质显著丰富在石头形成者中增加的蛋白质组.
结论:
- 氧化修饰的尿蛋白被认为是CaOx结石形成的关键病原性因素.
- 尿液中氧化蛋白质的水平增加是患结石的重要危险因素.
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