两性树枝状体辅助的反感核酸输送模仿了对大肠杆菌的抗核酸
Mariana Gomes1, Igor Resende1, Yana Zamoshchak2
1LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr Roberto Frias, 4200-465 Porto, Portugal; ALiCE - Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr Roberto Frias, 4200-465 Porto, Portugal.
两胞性树枝体有效地将反感核酸模仿物 (NAM) 传递给细菌,克服药物耐药性. 这种新的策略显示出强大的抗菌活性,并且在体内没有毒性.
科学领域:
- 生物技术是生物技术.
- 药物运输 药物运输 药物运输
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 抗菌素耐药性需要超越传统抗生素的新疗法策略.
- 反感核酸模仿剂 (NAMs) 提供精确的基因抑制,但面临着将其传递到细菌中的挑战.
研究的目的:
- 评估聚胺胺胺 (PAMAM) 两类树枝体 (ADs) 作为NAM的交付载体.
- 评估ADs增强NAM内化和抗菌活性对抗大肠杆菌的能力.
主要方法:
- 使用了两个具有不同氨基终端的PAMAMADs (1a和1b).
- 采用物理化学表征,流细胞计,显微镜,细菌活力测定和Galleria mellonella模型进行体内评估.
主要成果:
- 这两种抗药物都促进了细菌包膜的透和NAM内部化.
- 基于核酸 (PNA) 的 PAMAM AD 1b 配方与 NAM 实现了对大肠杆菌的 8 log10 减少.
- 在Galleria mellonella模型中,配方在三天内没有显示出毒性.
结论:
- 两形树枝体是基于PNA的NAM的有效传递载体.
- 这种方法是对抗抗菌素耐药性病原体的有希望的策略.
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